Bulmer J N, Hagin S V, Browne C M, Billington W D
Eur J Obstet Gynecol Reprod Biol. 1986 Oct;23(1-2):31-44. doi: 10.1016/0028-2243(86)90102-4.
The distribution of immunoglobulins in normal human endometrium throughout the menstrual cycle and in early pregnancy has been studied with an immunoperoxidase technique. In first-trimester decidua, IgG was detected within many cells of differing morphology and size. Large IgG-containing cells were often binucleate and were believed to be decidual cells. Examination of serial sections showed no kappa or lambda light-chain restriction, suggesting absorption of the immunoglobulin content. Medium-sized, irregular, IgG-containing cells were macrophages. An additional substantial population of small hyperchromatic IgG-containing cells were prominent around arterioles and adjacent to endometrial glands. From examination of adjacent sections stained with phloxine tartrazine, it was concluded that these represented endometrial granulocytes. Labelling for light chains again suggested absorption of the immunoglobulin content. In contrast, in non-pregnant endometrium immunoglobulin-containing stromal cells were uncommon, although IgG and IgA were detected in gland epithelium and secretions and in the stromal interstitium particularly in the secretory phase. These results support the notion that human endometrium lacks a classical secretory immune system and highlight the requirement for correlation between studies of cell surface markers, morphology and cell surface receptors.
利用免疫过氧化物酶技术，对正常人类子宫内膜在整个月经周期及妊娠早期免疫球蛋白的分布情况进行了研究。在孕早期蜕膜中，在许多形态和大小各异的细胞内检测到了IgG。含有大量IgG的细胞通常为双核，被认为是蜕膜细胞。连续切片检查显示不存在κ或λ轻链限制，提示免疫球蛋白含量被吸收。中等大小、形态不规则、含有IgG的细胞是巨噬细胞。另外，大量含IgG的小深染细胞在小动脉周围及子宫内膜腺体附近很突出。通过对用焰红 - 酒石黄染色的相邻切片进行检查得出结论，这些细胞代表子宫内膜粒细胞。轻链标记再次提示免疫球蛋白含量被吸收。相比之下，在非妊娠子宫内膜中，含免疫球蛋白的基质细胞并不常见，尽管在腺体上皮及分泌物中以及在基质间质中检测到了IgG和IgA，尤其在分泌期。这些结果支持了人类子宫内膜缺乏经典分泌免疫系统这一观点，并强调了细胞表面标志物、形态学及细胞表面受体研究之间相互关联的必要性。
