Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, USA.
J Ind Microbiol Biotechnol. 2019 Jul;46(7):993-1002. doi: 10.1007/s10295-019-02173-7. Epub 2019 Apr 9.
Biological H production has potential to address energy security and environmental concerns if produced from renewable or waste sources. The purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS produces H while oxidizing CO, a component of synthesis gas (Syngas). CO-linked H production is facilitated by an energy-converting hydrogenase (Ech), while a subsequent H oxidation reaction is catalyzed by a membrane-bound hydrogenase (MBH). Both hydrogenases contain [NiFe] active sites requiring 6 maturation factors (HypA-F) for assembly, but it is unclear which of the two annotated sets of hyp genes are required for each in R. gelatinosus CBS. Herein, we report correlated expression of hyp1 genes with Ech genes and hyp2 expression with MBH genes. Moreover, we find that while Ech H evolving activity is only delayed when hyp1 is deleted, hyp2 deletion completely disrupts MBH H uptake, providing a platform for a biologically driven water-gas shift reaction to produce H from CO.
如果能够从可再生资源或废物中生产，生物 H 生产具有解决能源安全和环境问题的潜力。紫色非硫光合细菌 Rubrivivax gelatinosus CBS 在氧化 CO（合成气（Syngas）的组成部分）的同时产生 H。通过能量转换氢化酶（Ech）促进 CO 相关 H 生产，而随后的 H 氧化反应由膜结合氢化酶（MBH）催化。两种氢化酶都含有[NiFe]活性位点，需要 6 个成熟因子（HypA-F）进行组装，但在 R. gelatinosus CBS 中，尚不清楚哪两组注释的 hyp 基因分别需要这两种酶。在此，我们报告了 hyp1 基因与 Ech 基因的相关表达以及 hyp2 表达与 MBH 基因的相关表达。此外，我们发现，虽然 Ech H 进化活性仅在 hyp1 缺失时延迟，但 hyp2 缺失完全破坏了 MBH H 的摄取，为从 CO 生产 H 的生物驱动水煤气变换反应提供了平台。
