Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO, USA.
Dalton Trans. 2009 Dec 7(45):9970-8. doi: 10.1039/b913426n. Epub 2009 Oct 27.
This review focuses on recent progress in developing heterologous and recombinant expression as well as in vitro maturation systems for the biosynthesis of active [FeFe] and [NiFe]-hydrogenases, which catalyze the reversible reaction, H2 <--> 2e- + 2H+. Activities of [FeFe] and [NiFe]-hydrogenases produced from different recombinant and in vitro maturation approaches are compared. Examples of how hydrogenase expression supports basic and applied studies of these enzymes are presented, and barriers to achieving more viable biological and synthetic H2-production systems and catalysts are addressed.
本文综述了用于生物合成活性 [FeFe] 和 [NiFe]-氢化酶的异源和重组表达以及体外成熟系统的最新进展，[FeFe] 和 [NiFe]-氢化酶催化可逆反应 H2 <--> 2e- + 2H+。比较了不同重组和体外成熟方法生产的 [FeFe] 和 [NiFe]-氢化酶的活性。介绍了氢化酶表达如何支持这些酶的基础和应用研究的实例，并讨论了实现更可行的生物和合成 H2 生产系统和催化剂的障碍。
