Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
PLoS One. 2021 Aug 6;16(8):e0255899. doi: 10.1371/journal.pone.0255899. eCollection 2021.
Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 β-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.
低含量人参皂苷（MGs）（包括人参皂苷 F2、化合物 K、PPT 等）通常不是人参植物自然产生的，而是通过对主要人参皂苷进行去糖基化得到的。然而，用于生产去糖基化人参皂苷的传统方法主要集中在利用肠道微生物进行转化。在这项研究中，采用平板显色法从 161 种来源于野山参的产β-葡萄糖苷酶土壤微生物中筛选出一种可食用药用真菌——裂蹄木层孔菌 JE0512。此外，使用 20g 玉米麸皮作为基质、4g 人参提取物（GE）和 20%接种量，在固态发酵（SSF）中使用裂蹄木层孔菌 JE0512 从人参提取物（GE）中生产 CK，结果表明 CK 含量最高可达 29.13mg/g。裂蹄木层孔菌 JE0512 与纤维素酶（黑曲霉）结合后，最终产物中 MGs（F2、CK 和 PPT）的含量从 1.66mg/g 增加到 130.79mg/g。我们的研究结果表明，裂蹄木层孔菌属具有将 GE 生物转化为 CK 的潜力，裂蹄木层孔菌 JE0512 与纤维素酶的结合可能为从 GE 生产 MGs 铺平道路。
