1 Public Health Laboratories, Washington State Department of Health, Shoreline, Washington.
2 Communicable Disease Epidemiology, Washington State Department of Health, Shoreline, Washington.
Foodborne Pathog Dis. 2019 Jul;16(7):513-523. doi: 10.1089/fpd.2018.2592. Epub 2019 Apr 10.
The Washington State Department of Health Public Health Laboratories (WAPHL) has tested 11,501 samples between 2007 and 2017 for a foodborne disease using a combination of identification, serotyping, and subtyping tools. During this period there were 8037 total clinical and environmental samples tested by pulsed-field gel electrophoresis (PFGE), including 512 foodborne disease clusters and 2176 PFGE patterns of subsp. . There were 2446 Shiga toxin-producing samples tested by PFGE, which included 158 foodborne disease clusters and 1174 PFGE patterns. There were 332 samples of tested by PFGE, including 35 foodborne disease clusters and 104 PFGE patterns. Sources linked to outbreaks included raw chicken, unpasteurized dairy products, various produce types, and undercooked beef among others. As next-generation sequencing (NGS) replaces PFGE, the impact of this transition is expected to be significant given the enhanced cluster detection power NGS brings. The measures presented here will be a reference baseline in future years.
华盛顿州公共卫生实验室（WAPHL）在 2007 年至 2017 年期间使用鉴定、血清分型和亚型分型工具组合测试了 11501 个食源性疾病样本。在此期间，共通过脉冲场凝胶电泳（PFGE）测试了 8037 个临床和环境样本，包括 512 个食源性疾病群和 2176 个亚群的 PFGE 图谱。有 2446 个产志贺毒素的通过 PFGE 测试，其中包括 158 个食源性疾病群和 1174 个 PFGE 图谱。有 332 个通过 PFGE 测试，包括 35 个食源性疾病群和 104 个 PFGE 图谱。暴发相关的来源包括生鸡肉、未经巴氏消毒的乳制品、各种农产品以及未煮熟的牛肉等。随着下一代测序（NGS）取代 PFGE，鉴于 NGS 带来的增强的聚类检测能力，这种转变的影响预计将是重大的。本文提出的措施将是未来几年的参考基准。
