McIntyre G, Stanford J L
J Appl Bacteriol. 1986 Oct;61(4):295-8. doi: 10.1111/j.1365-2672.1986.tb04290.x.
Antigenic analysis by immunodiffusion has been applied to 74 strains of mycobactin-dependent mycobacteria. Thirty-eight strains were of Mycobacterium paratuberculosis from cases of Johne's disease of cattle or goats. The remaining cultures were obtained from a variety of animals and included the wood pigeon bacillus. Rabbit antisera were raised to some of the strains and these, together with antisera to M. avium and M. intracellulare, were used to examine sonicate preparations of all the cultures. All were found to be antigenically identical with M. avium and none were found to belong to M. intracellulare. A predominance of the cultures from Johne's disease belonged to the potential brunense subspecies of M. avium, and the remainder together with the majority of the other mycobactin-dependent strains belonged to the type subspecies. In view of these findings the separate species status of M. paratuberculosis is refuted and some difficulty remains in the nomenclature of strains giving rise to Johne's disease.
通过免疫扩散进行的抗原分析已应用于74株依赖分枝杆菌素的分枝杆菌。38株来自牛或山羊副结核病病例的副结核分枝杆菌。其余培养物来自多种动物，包括林鸽杆菌。用一些菌株制备了兔抗血清，这些抗血清与鸟分枝杆菌和胞内分枝杆菌的抗血清一起，用于检测所有培养物的超声提取物。结果发现所有菌株在抗原性上均与鸟分枝杆菌相同，未发现属于胞内分枝杆菌的菌株。来自副结核病的培养物中，大多数属于鸟分枝杆菌潜在的布鲁内斯亚种，其余的以及大多数其他依赖分枝杆菌素的菌株属于典型亚种。鉴于这些发现，副结核分枝杆菌的独立种地位被否定，在引起副结核病的菌株命名方面仍存在一些困难。
