在生长过程中使用枯草芽孢杆菌枯草杆菌蛋白酶信号肽高效分泌TEMβ-内酰胺酶。
Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM beta-lactamase during growth.
作者信息
Wong S L, Kawamura F, Doi R H
出版信息
J Bacteriol. 1986 Nov;168(2):1005-9. doi: 10.1128/jb.168.2.1005-1009.1986.
Abstract
We report the development of an efficient Bacillus subtilis secretory system, with the secreted product stably maintained in the medium for 100 h. The system is based on characterization of the subtilisin signal peptidase cleavage site and promoters, catabolite repression of sporulation, presence of a vegetative secreting mechanism, and availability of a protease-deficient strain.
摘要
我们报道了一种高效的枯草芽孢杆菌分泌系统的开发，其分泌产物可在培养基中稳定维持100小时。该系统基于枯草杆菌蛋白酶信号肽酶切割位点和启动子的特性、孢子形成的分解代谢物阻遏、营养分泌机制的存在以及蛋白酶缺陷菌株的可用性。
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