Siebert Jonathan T, Kivel Alexey N, Atkinson Liam P, Stevens Tim J, Laue Ernest D, Virnau Peter
Department of Physics, Johannes Gutenberg University Mainz, Staudinger Weg 9, 55128 Mainz, Germany.
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
Polymers (Basel). 2017 Aug 2;9(8):317. doi: 10.3390/polym9080317.
Recent developments have for the first time allowed the determination of three-dimensional structures of individual chromosomes and genomes in nuclei of single haploid mouse embryonic stem (ES) cells based on Hi⁻C chromosome conformation contact data. Although these first structures have a relatively low resolution, they provide the first experimental data that can be used to study chromosome and intact genome folding. Here we further analyze these structures and provide the first evidence that G1 phase chromosomes are knotted, consistent with the fact that plots of contact probability vs sequence separation show a power law dependence that is intermediate between that of a fractal globule and an equilibrium structure.
最近的进展首次使得基于Hi-C染色体构象接触数据确定单倍体小鼠胚胎干细胞细胞核中单个染色体和基因组的三维结构成为可能。尽管这些最初的结构分辨率相对较低，但它们提供了首批可用于研究染色体和完整基因组折叠的实验数据。在这里，我们进一步分析这些结构，并提供了首个证据表明G1期染色体是打结的，这与接触概率与序列间隔的关系图显示出幂律依赖性这一事实一致，该幂律依赖性介于分形球状体和平衡结构之间。
