Lorusso J R, Moffat S, Ohman J L
J Allergy Clin Immunol. 1986 Nov;78(5 Pt 1):928-37. doi: 10.1016/0091-6749(86)90242-3.
Rabbit antiserum to the mouse major urinary protein identified a single antigen that was also found in mouse serum and pelt extract. The skin test reactivity of mouse-pelt extract and mouse urine in two mouse-allergic subjects was significantly reduced after immunoabsorption with the gamma globulin fraction of this antiserum. The antigen defined by this antiserum was designated mouse allergen 1 (MA1). An immunoelectrophoretic procedure was set up to measure its concentration. MA1 had a molecular weight of approximately 19,000 on Sephadex gel filtration and 18,000 to 21,000 on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Isoelectric focusing identified at least four bands with antigenic activity; the major band had an isoelectric point of 3.9. Significant antigenic and allergenic activity of MA1 was retained on reduction and digestion with papain and pepsin. Heating at 90 degrees C for periods up to 180 minutes resulted in a progressive loss, but not abolition, of activity. Serum and urine derived from male mice contained approximately fourfold more MA1 than samples derived from female mice. Urine contained at least 100-fold more MA1 than serum. Of the tissue extracts studied, liver extract had the highest amount of MA1. The immunochemical properties of MA1, its tissue distribution, and sex differences in its concentration provide strong evidence that MA1 is identical to the previously described mouse major urinary protein.
针对小鼠主要尿蛋白的兔抗血清识别出一种单一抗原，该抗原也存在于小鼠血清和皮毛提取物中。用这种抗血清的γ球蛋白组分进行免疫吸附后，两名对小鼠过敏的受试者对小鼠皮毛提取物和小鼠尿液的皮肤试验反应性显著降低。这种抗血清所定义的抗原被命名为小鼠变应原1（MA1）。建立了一种免疫电泳方法来测定其浓度。在葡聚糖凝胶过滤中，MA1的分子量约为19,000，在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中为18,000至21,000。等电聚焦鉴定出至少四条具有抗原活性的条带；主要条带的等电点为3.9。用木瓜蛋白酶和胃蛋白酶还原和消化后，MA1仍保留显著的抗原和变应原活性。在90℃加热长达180分钟会导致活性逐渐丧失，但不会完全消除。雄性小鼠的血清和尿液中所含的MA1约为雌性小鼠样本的四倍。尿液中所含的MA1比血清至少多100倍。在所研究的组织提取物中，肝脏提取物中的MA1含量最高。MA1的免疫化学特性、其组织分布以及其浓度的性别差异提供了有力证据，表明MA1与先前描述的小鼠主要尿蛋白相同。
