Konieczny A, Morgenstern J P, Bizinkauskas C B, Lilley C H, Brauer A W, Bond J F, Aalberse R C, Wallner B P, Kasaian M T
ImmuLogic Pharmaceutical Corporation, Waltham MA 02154, USA.
Immunology. 1997 Dec;92(4):577-86. doi: 10.1046/j.1365-2567.1997.00386.x.
Canis familiaris allergen 1 (Can f 1) and Canis familiaris allergen 2 (Can f 2) are the two major allergens present in dog dander extracts. We now report the isolation of cDNAs encoding both proteins and present their nucleotide and deduced amino acid sequences. Can f 1, produced by tongue epithelial tissue, has homology with the von Ebner's gland (VEG) protein, a salivary protein not previously thought to have allergenic properties. Can f 2, produced by tongue and parotid gland, has homology with mouse urinary protein (MUP), a known allergen. Both VEG protein and MUP are members of the lipocalin family of small ligand-binding proteins. Recombinant forms of Can f 1 and Can f 2 were produced and tested for immunoglobulin E (IgE) reactivity. Among dog-allergic subjects, 45% had IgE directed exclusively to rCan f 1, and 25% had IgE to both rCan f 1 and rCan f 2. In addition, both recombinant proteins were able to cross-link IgE and elicit histamine release from peripheral blood leucocytes in vitro. These findings confirm that Can f 1 and Can f 2 are major and minor dog allergens, respectively, and demonstrate that recombinant forms of dog allergens retain at least some IgE-binding epitopes.
犬过敏原1（Can f 1）和犬过敏原2（Can f 2）是犬皮屑提取物中的两种主要过敏原。我们现在报告编码这两种蛋白质的cDNA的分离，并呈现它们的核苷酸和推导的氨基酸序列。由舌上皮组织产生的Can f 1与埃伯纳腺（VEG）蛋白具有同源性，VEG蛋白是一种唾液蛋白，以前认为不具有致敏特性。由舌和腮腺产生的Can f 2与小鼠尿蛋白（MUP）具有同源性，MUP是一种已知的过敏原。VEG蛋白和MUP都是小配体结合蛋白脂钙蛋白家族的成员。制备了Can f 1和Can f 2的重组形式，并检测了它们与免疫球蛋白E（IgE）的反应性。在对犬过敏的受试者中，45%的人IgE仅针对重组Can f 1，25%的人IgE针对重组Can f 1和重组Can f 2两者。此外，两种重组蛋白都能够交联IgE并在体外引起外周血白细胞释放组胺。这些发现证实Can f 1和Can f 2分别是主要和次要的犬过敏原，并表明犬过敏原的重组形式至少保留了一些IgE结合表位。
