Araújo Góis Morais Paulo Leonardo, García-Amado María, Lima Ruthnaldo Rodrigues Melo, Córdoba-Claros Angélica, Souza Cavalcante Jeferson, Clascá Francisco, Nascimento Expedito Silva
Department of Morphology, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
Department of Anatomy & Neuroscience, School of Medicine, Autonoma de Madrid University, Madrid, Spain.
Front Neuroanat. 2019 Mar 27;13:36. doi: 10.3389/fnana.2019.00036. eCollection 2019.
The amygdaloid complex (AC) is a heterogeneous aggregate of nuclei located in the rostromedial region of the temporal lobe. In addition to being partly connected among themselves, the AC nuclei are strongly interconnected with the cerebral cortex, striatum, basal forebrain, hypothalamus and brainstem. Animal and human functional studies have established that the AC is a central hub of the neuronal networks supporting emotional responsivity, particularly its negative/aversive components. Dysfunction of AC circuits in humans has been implicated in anxiety, depression, schizophrenia and bipolar disorder. The small New-World marmoset monkey has recently become a key model for neuroscience research. However, the nuclear and fiber tract organization of marmoset AC has not been examined in detail. Thus, the extent to which it can be compared to the AC of Old-World (human and macaque) primates is yet unclear. Here, using Nissl and acetylcholinesterase (AChE) histochemical stains as a reference, we analyzed the cytoarchitecture and nuclear parcellation of the marmoset AC. In addition, given the increasing relevance of tractographic localization for high-resolution imaging studies in non-human primates, we also identified the myelin fiber tracts present within and around the AC as revealed by the Gallyas method. The present study provides a detailed atlas of marmoset AC. Moreover, it reveals that, despite phylogenetic distance and brain size differences, every nucleus and myelinated axon bundle described in human and macaque studies can be confidently recognized in marmosets.
杏仁复合体（AC）是位于颞叶嘴内侧区域的一组异质性核团。AC核团除了部分相互连接外，还与大脑皮层、纹状体、基底前脑、下丘脑和脑干紧密相连。动物和人类功能研究已证实，AC是支持情绪反应，尤其是其负面/厌恶成分的神经网络的核心枢纽。人类AC回路功能障碍与焦虑症、抑郁症、精神分裂症和双相情感障碍有关。小型新大陆狨猴最近已成为神经科学研究的关键模型。然而，狨猴AC的核团和纤维束组织尚未得到详细研究。因此，它与旧大陆（人类和猕猴）灵长类动物的AC相比能达到何种程度尚不清楚。在这里，我们以尼氏染色和乙酰胆碱酯酶（AChE）组织化学染色作为参考，分析了狨猴AC的细胞结构和核团划分。此外，鉴于纤维束追踪定位在非人类灵长类动物高分辨率成像研究中的相关性日益增加，我们还通过加利亚斯方法确定了AC内部和周围存在的髓鞘纤维束。本研究提供了一份狨猴AC的详细图谱。此外，研究表明，尽管存在系统发育距离和脑大小差异，但在人类和猕猴研究中描述的每个核团和有髓轴突束在狨猴中都能得到确切识别。
