Mahmoud Al Zoubi Omar
Pak J Biol Sci. 2019 Jan;22(2):73-82. doi: 10.3923/pjbs.2019.73.82.
Commiphora gileadensis a medicinal plant rare species. A large amount of plant materials were needed to produce secondary metabolite under in vitro culture. Therefore, callus is used in the in vitro culture, since it can proliferate quickly and continuously provide an appropriate amount of plant which used for extracting the antimicrobial compounds from C. gileadensis.
Rapid protocol for optimum callus production has been assessed to overcome limitations of the conventional propagation methods. The effect of plant growth regulator (PGR) on the regeneration of C. gileadensis was investigated for callus induction experiment using a standard MS medium with various concentrations of 6-Benzyl adenine (BA), Kinetin (Kn), 2,4-dichlorophenoxy acetic acid (2,4-D) and naphthalene acetic acid (NAA) at 0.0, 0.5, 1.0,1.5, 2.0 and 2.5 mg L-1.
The result showed that the maximum regeneration of callus induced the fresh and dry weight were obtained 5675±1321 and 376.7±56.9 mg, respectively on MS media containing 2 mg L-1 2,4D + 0.5 mg L-1 BA after 12 weeks. The anti-bacterial and anti-fungal activities of C. gileadensis were evaluated using the callus and ex vitro extracts, six bacterial species fungal genera were used the agar well diffusion method used of 25, 50, 75 and 100 μL methanolic or ethonlic extracts of ex vitro and callus had considerable inhibition effects on the tested bacteria and fungi.
Callus culture technique may be an important tool to get the C. gileadensis quickly as compared to the natural growth phenomenon where it takes many years. Moreover, it's give us an opportunity to get the active constituent without destroying the plant available in nature. The results of the present study can improve our understanding of the economic importance of C. gileadensis as activity ingredient antimicrobial agent and provided methods for its preparation.
乳香黄连木是一种珍稀药用植物。在体外培养条件下，需要大量植物材料来生产次生代谢产物。因此，愈伤组织被用于体外培养，因为它能够快速增殖，并持续提供适量的植物材料，用于从乳香黄连木中提取抗菌化合物。
为克服传统繁殖方法的局限性，评估了优化愈伤组织生产的快速方案。使用添加了不同浓度（0.0、0.5、1.0、1.5、2.0和2.5 mg L-1）的6-苄基腺嘌呤（BA）、激动素（Kn）、2,4-二氯苯氧乙酸（2,4-D）和萘乙酸（NAA）的标准MS培养基，研究植物生长调节剂（PGR）对乳香黄连木再生的影响，进行愈伤组织诱导实验。
结果表明，在含有2 mg L-1 2,4-D + 0.5 mg L-1 BA的MS培养基上培养12周后，愈伤组织的最大再生量分别为鲜重5675±1321 mg和干重376.7±56.9 mg。使用愈伤组织和离体提取物评估了乳香黄连木的抗菌和抗真菌活性，采用琼脂扩散法，用六种细菌和真菌属，25、50、75和100 μL离体和愈伤组织的甲醇或乙醇提取物对受试细菌和真菌有显著抑制作用。
与需要多年时间的自然生长现象相比，愈伤组织培养技术可能是快速获得乳香黄连木的重要工具。此外，它为我们提供了一个在不破坏自然界现有植物的情况下获取活性成分的机会。本研究结果可以增进我们对乳香黄连木作为活性成分抗菌剂的经济重要性的理解，并提供其制备方法。
