Waleron Malgorzata, Misztak Agnieszka, Waleron Michal, Jonca Joanna, Furmaniak Magda, Waleron Krzysztof
1Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Abrahama, Poland.
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland.
Int J Syst Evol Microbiol. 2019 Jun;69(6):1751-1759. doi: 10.1099/ijsem.0.003387. Epub 2019 Apr 11.
Gram-stain-negative, rod-shaped pectinolytic bacteria strains designated as DPMP315, DPMP316, DPMP317 and DPMP318 isolated from groundwater sampled from a vegetable field in the North of Poland, were subjected to the polyphasic analyses. Multilocus sequence analyses based on five housekeeping genes (gyrA, recA, recN, rpoA and rpoS) revealed their distinctiveness from the other species of the genus, simultaneously indicating that the newly described species, Pectobacterium punjabense, as well as Pectobacterium parmentieri and P. wasabiae, to be the closest relatives. In silico DNA-DNA hybridization (<43.1 %) and average nucleotide identity (<92.5 %) values of strain DPMP315 with other type strains of species of the genus Pectobacterium supported the delineation of the novel strain as representing a novel species. The phenotypic comparisons, fatty acid methyl esters compositions, genetic rep PCR fingerprint and detailed whole-cell MALDI-TOF mass spectrometry proteomic profiles permitted the differentiation of Polish strains from the type strains of all other known species of the genus Pectobacterium. The results of polyphasic analyses performed for four Polish strains are the basis for the distinction of the novel species. Here, we propose to establish DPMP315 as a type strain (=PCM3006=LMG 31077) with the name Pectobacterium polonicum sp. nov.
从波兰北部一个蔬菜地采集的地下水中分离出的革兰氏染色阴性、杆状的果胶分解细菌菌株DPMP315、DPMP316、DPMP317和DPMP318，进行了多相分析。基于五个管家基因（gyrA、recA、recN、rpoA和rpoS）的多位点序列分析揭示了它们与该属其他物种的差异，同时表明新描述的物种旁遮普果胶杆菌（Pectobacterium punjabense）以及胡萝卜果胶杆菌（Pectobacterium parmentieri）和山葵果胶杆菌（P. wasabiae）是其最亲近的亲缘种。菌株DPMP315与果胶杆菌属其他物种模式菌株的电子DNA-DNA杂交（<43.1%）和平均核苷酸同一性（<92.5%）值支持将该新菌株划定为代表一个新物种。表型比较、脂肪酸甲酯组成、遗传rep PCR指纹图谱和详细的全细胞MALDI-TOF质谱蛋白质组图谱允许区分波兰菌株与果胶杆菌属所有其他已知物种的模式菌株。对四个波兰菌株进行的多相分析结果是区分该新物种的基础。在此，我们提议将DPMP315确立为模式菌株（=PCM3006=LMG 31077），命名为波兰果胶杆菌（Pectobacterium polonicum sp. nov.）。
