US Department of Agriculture, US National Poultry Research Center, Athens, GA 30605,.
Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD 20740.
Genomics. 2020 Jan;112(1):528-544. doi: 10.1016/j.ygeno.2019.04.005. Epub 2019 Apr 8.
A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995-1998. Isolates separated into two genomic clades and 12 subgroups due to 742 mutations. Nineteen ORF-disruptive mutations were found, and in addition, bigA had exceptional heterogeneity requiring additional evaluation. The TRAMS algorithm detected only 6 ORF disruptions. The sefD mutation was the most frequently encountered mutation and it was prevalent in human, poultry, environmental and mouse isolates. These results confirm previous assessments of the mouse as a rich source of Salmonella enterica serovar Enteritidis that varies in genotype and phenotype.
本研究共分析了 91 株来自宾夕法尼亚州家禽养殖场中捕获的野生鼠伤寒沙门氏菌肠炎亚种分离株的基因组草图序列。本研究的目的之一是寻找破坏开放阅读框（ORF）的突变，另一个目的是确定是否存在于从其他来源获得的分离株中的 ORF 破坏性突变。1995-1998 年间共获得了 83 只老鼠。由于 742 个突变，这些分离株被分成了两个基因组分支和 12 个亚群。共发现了 19 个 ORF 破坏性突变，此外，bigA 存在特殊的异质性，需要进一步评估。TRAMS 算法仅检测到 6 个 ORF 中断。sefD 突变是最常见的突变，它普遍存在于人类、家禽、环境和鼠伤寒沙门氏菌肠炎亚种的分离株中。这些结果证实了先前对鼠作为肠炎沙门氏菌肠炎亚种丰富来源的评估，该菌在基因型和表型上存在差异。
