Xia Ying, Li Hao, Shen Yaoqin
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Front Vet Sci. 2020 Aug 4;7:463. doi: 10.3389/fvets.2020.00463. eCollection 2020.
The growing consumption of snakes in China has led to a boom in edible snakes farming. Food producing reptiles, such as snakes can carry many pathogenic microbes and potentially infect humans. Here, we report the occurrence of multi drug resistant strains isolated from edible snakes in China. Our results showed that the isolated was resistant to the majority of the tested drugs and sensitive to tetracycline and amikacin. Antimicrobial susceptibility test showed that the strains carried the blaTEM, qnrD, aadA, catA o, sul I, and sul II genes. The pathogenicity testing of the isolated strains showed that these strains were highly pathogenic (75% mortality, with LD at 10 CFU/mL). The chickens in the high-dose groups developed acute septicemia and died within 24 h. Results of the dissection showed extensive abdominal bleeding and swelling in the high dose groups, as well as hyperemia edema in the livers, lungs, kidneys, cecum, and bursa of the chickens, with spotty bleeding. In addition, rod-shaped bacterial aggregation was also seen in the visual field. A total of 23 virulence genes, mainly associated with pathogenicity island were tested, of which 8 genes including avrA, iacP, prgK, ssrA, siiD (spi4D), siiE, spi4H, and pipC were found positive. Altogether, our results provide useful information regarding edible snakes contaminated with , which may have public health implications.
在中国，蛇类消费的不断增长导致食用蛇养殖蓬勃发展。作为食物来源的爬行动物，如蛇，可能携带多种致病微生物并有可能感染人类。在此，我们报告在中国从食用蛇中分离出多重耐药菌株的情况。我们的结果表明，分离出的菌株对大多数测试药物耐药，而对四环素和阿米卡星敏感。药敏试验表明，这些菌株携带blaTEM、qnrD、aadA、catA o、sul I和sul II基因。对分离菌株的致病性测试表明，这些菌株具有高度致病性（死亡率为75%，半数致死剂量为10 CFU/mL）。高剂量组的鸡发生急性败血症并在24小时内死亡。解剖结果显示，高剂量组鸡的腹部有广泛出血和肿胀，肝脏、肺、肾、盲肠和法氏囊有充血水肿，并伴有点状出血。此外，在视野中还可见杆状细菌聚集。总共检测了23个主要与致病岛相关的毒力基因，其中包括avrA、iacP、prgK、ssrA、siiD（spi4D）、siiE、spi4H和pipC在内的8个基因呈阳性。总之，我们的结果提供了有关被污染的食用蛇的有用信息，这可能对公共卫生有影响。
