Department of State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.
Phytochemistry. 2019 Jul;163:1-10. doi: 10.1016/j.phytochem.2019.03.026. Epub 2019 Apr 8.
Herein, five polyphenol oxidases (PPOs) obtained from Morus notabilis (Mn) were characterized. Chlorogenic acid was the most readily oxidized substrate by these MnPPOs, and the products derived from the oxidation of chlorogenic acid by MnPPOs were tested for antimicrobial activity. The results showed that products of the five MnPPOs exhibited good inhibitory effects against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Sclerotinia sclerotiorum, and Botrytis cinerea. Because the products of MnPPO1 exhibited the strongest antimicrobial activity, the antimicrobial mechanism of these products was explored. The results showed that the products of MnPPO1 increased cell membrane permeability and chitinase and β-1,3-glucanase activities.
本文对从桑（Morus notabilis）中获得的 5 种多酚氧化酶（PPO）进行了表征。这些 MnPPOs 最容易氧化绿原酸，并且测试了 MnPPOs 氧化绿原酸产生的产物的抗菌活性。结果表明，这 5 种 MnPPOs 的产物对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、核盘菌和灰葡萄孢有很好的抑制作用。由于 MnPPO1 的产物表现出最强的抗菌活性，因此探索了这些产物的抗菌机制。结果表明，MnPPO1 的产物增加了细胞膜的通透性和几丁质酶和β-1,3-葡聚糖酶的活性。
