College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
School of Automation and Information Engineering, Sichuan University of Science and Engineering, Zigong 643000, China.
Molecules. 2019 Apr 10;24(7):1401. doi: 10.3390/molecules24071401.
Magnetite (Fe₃O₄) nanoparticles are widely used in multiple biomedical applications due to their magnetic properties depending on the size, shape and organization of the crystals. However, the crystal growth and morphology of Fe₃O₄ nanoparticles remain difficult to control without using organic solvent or a high temperature. Inspired by the natural biomineralization process, a 14-mer bi-functional copolypeptide, leveraging the affinity of binding Fe₃O₄ together with targeting ovarian cancer cell A2780, was used as a template in the biomimetic mineralization of magnetite. Alongside this, a ginger extract was applied as an antioxidant and a size-conditioning agent of Fe₃O₄ crystals. As a result of the cooperative effects of the peptide and the ginger extract, the size and dispersibility of Fe₃O₄ were controlled based on the interaction of the amino acid and the ginger extract. Our study also demonstrated that the obtained particles with superparamagnetism could selectively be taken up by A2780 cells. In summary, the Fe₃O₄-QY-G nanoparticles may have potential applications in targeting tumor therapy or angiography.
磁铁矿（Fe3O4）纳米粒子由于其尺寸、形状和晶体结构的不同而具有磁性，因此被广泛应用于多种生物医学领域。然而，如果不使用有机溶剂或高温，Fe3O4 纳米粒子的晶体生长和形态仍然难以控制。受天然生物矿化过程的启发，我们使用了一种 14 mer 双功能共聚多肽，该多肽利用与 Fe3O4 结合的亲和力以及对卵巢癌细胞 A2780 的靶向性，作为仿生矿化磁铁矿的模板。此外，姜提取物被用作抗氧化剂和 Fe3O4 晶体的尺寸调节剂。由于多肽和姜提取物的协同作用，基于氨基酸和姜提取物的相互作用，控制了 Fe3O4 的尺寸和分散性。我们的研究还表明，具有超顺磁性的所得颗粒可以被 A2780 细胞选择性摄取。总之，Fe3O4-QY-G 纳米颗粒可能在肿瘤靶向治疗或血管造影中有潜在的应用。
