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在 中,对植物激素处理和非生物胁迫响应的基因家族进行全基因组特征描述、进化和表达谱分析。

Genome-Wide Characterization, Evolution, and Expression Profiling of Gene Family in Response to Phytohormone Treatments and Abiotic Stress in .

机构信息

Key Laboratory of State Forestry Administration on Tropical Forest Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China.

出版信息

Int J Mol Sci. 2019 Apr 10;20(7):1765. doi: 10.3390/ijms20071765.

DOI:10.3390/ijms20071765
PMID:30974801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6480042/
Abstract

genes play important roles in plant development, growth, and stress responses. However, little information regarding the functions of genes is available for . In our study, genome-wide characterization and identification of genes were performed in . Results showed that 27 genes, which divided into seven sub-families (I-VII), were found, and all but two genes showed no intron by gene structure and conserved motif analysis. To further identify the function of EgrVQ proteins, gene expression analyses were also developed under hormone treatments (brassinosteroids, methyl jasmonate, salicylic acid, and abscisic acid) and abiotic conditions (salt stress, cold 4 °C, and heat 42 °C). The results of a quantitative real-time PCR analysis indicated that the were variously expressed under different hormone treatments and abiotic stressors. Our study provides a comprehensive overview of genes in , which will be beneficial in the molecular breeding of to promote its resistance to abiotic stressors; the results also provide a basis from which to conduct further investigation into the functions of genes in .

摘要

基因在植物的发育、生长和应激反应中起着重要作用。然而,对于 ,关于基因功能的信息却很少。在我们的研究中,在 中进行了全基因组的特征描述和 基因的鉴定。结果表明,发现了 27 个 基因,它们分为七个亚家族(I-VII),除了两个 基因外,通过基因结构和保守基序分析,所有 基因都没有内含子。为了进一步鉴定 EgrVQ 蛋白的功能,还在激素处理(油菜素内酯、茉莉酸甲酯、水杨酸和脱落酸)和非生物条件(盐胁迫、4°C 低温和 42°C 高温)下进行了基因表达分析。实时定量 PCR 分析的结果表明,在不同的激素处理和非生物胁迫下, 基因的表达情况各不相同。我们的研究提供了 中 基因的全面概述,这将有助于对 进行分子育种,以提高其对非生物胁迫的抗性;研究结果还为进一步研究 基因在 中的功能提供了基础。

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