College of Urban Construction, Zhejiang Shuren University, Hangzhou, 310015, China.
Key Laboratory of State Forestry Administration on Tropical Forestry, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China.
BMC Plant Biol. 2024 Jun 18;24(1):573. doi: 10.1186/s12870-024-05288-x.
The GRAS gene family is a class of plant-specific transcription factors with important roles in many biological processes, such as signal transduction, disease resistance and stress tolerance, plant growth and development. So far, no information available describes the functions of the GRAS genes in Eucalyptus grandis.
A total of 82 GRAS genes were identified with amino acid lengths ranging from 267 to 817 aa, and most EgrGRAS genes had one exon. Members of the GRAS gene family of Eucalyptus grandis are divided into 9 subfamilies with different protein structures, while members of the same subfamily have similar gene structures and conserved motifs. Moreover, these EgrGRAS genes expanded primarily due to segmental duplication. In addition, cis-acting element analysis showed that this family of genes was involved involved in the signal transduction of various plant hormones, growth and development, and stress response. The qRT-PCR data indicated that 18 EgrGRAS genes significantly responded to hormonal and abiotic stresses. Among them, the expression of EgrGRAS13, EgrGRAS68 and EgrGRAS55 genes was significantly up-regulated during the treatment period, and it was hypothesised that members of the EgrGRAS family play an important role in stress tolerance.
In this study, the phylogenetic relationship, conserved domains, cis-elements and expression patterns of GRAS gene family of Eucalyptus grandis were analyzed, which filled the gap in the identification of GRAS gene family of Eucalyptus grandis and laid the foundation for analyzing the function of EgrGRAS gene in hormone and stress response.
GRAS 基因家族是一类植物特异性转录因子，在许多生物学过程中发挥着重要作用，如信号转导、抗病性和抗逆性、植物生长发育等。迄今为止，尚无关于 GRAS 基因在桉树中的功能的信息。
共鉴定出 82 个 GRAS 基因，其氨基酸长度从 267 到 817 个氨基酸不等，大多数 EgrGRAS 基因只有一个外显子。桉树 GRAS 基因家族的成员分为 9 个子家族，具有不同的蛋白质结构，而同一子家族的成员具有相似的基因结构和保守基序。此外，这些 EgrGRAS 基因主要通过片段复制扩增。此外，顺式作用元件分析表明，该家族基因参与了多种植物激素、生长发育和应激反应的信号转导。qRT-PCR 数据表明，18 个 EgrGRAS 基因对激素和非生物胁迫有显著响应。其中，EgrGRAS13、EgrGRAS68 和 EgrGRAS55 基因在处理期的表达显著上调，推测 EgrGRAS 家族成员在耐胁迫中发挥重要作用。
本研究分析了桉树 GRAS 基因家族的系统发育关系、保守结构域、顺式元件和表达模式，填补了桉树 GRAS 基因家族鉴定的空白，为分析 EgrGRAS 基因在激素和胁迫响应中的功能奠定了基础。
