Dharmawati I Gusti Agung Ayu, Mahadewa Tjokorda Gde Bagus, Widyadharma I Putu Eka
Doctoral Program, Faculty of Medicine, Udayana University, Bali, Indonesia.
Neurosurgery Department, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, Indonesia.
Open Access Maced J Med Sci. 2019 Mar 28;7(6):1032-1036. doi: 10.3889/oamjms.2019.222. eCollection 2019 Mar 30.
The purpose of this study was to determine the antibacterial activity of earthworms through inhibitory zone diameter to the growth of the bacterium as the cause of periodontitis.
This was an experimental study with randomised posttest-only control group design. The study was conducted at the Microbiology Research Center laboratory at the Faculty of Dentistry, Airlangga University, Indonesia. The study was conducted in vitro, the sample size was calculated using the Federer formula as many as four agar plates containing bacteria , with each plate given five different treatments: control (ethanol), earthworm extract (ECT) with concentrations of 50%, 25%, 12.5%, and 6.25% respectively. The data in the form of inhibition zone diameter (measured in millimetres) obtained were tested using One-Way ANOVA.
The mean diameter of the inhibitory zone extract of earthworm on the growth of Phorphyromonas gingivalis bacteria in the treatment group had significant differences (p < 0.05). The mean inhibition zones between controls and the ECT treatment group (ECT 50%, ECT 25%, ECT 12.5%) were statistically different (p < 0.05), in contrast with ECT 6.25% (p > 0.05) which did not show significant difference with the control group (p > 0.05).
earthworm extract with a concentration of 50% has the largest diameter of the inhibitory zone on the growth of the bacteria. The 6.25% earthworm extract showed no antibacterial activity against the growth of bacteria.
本研究旨在通过抑制圈直径来确定蚯蚓对作为牙周炎病因的细菌生长的抗菌活性。
这是一项采用仅后测随机对照组设计的实验研究。该研究在印度尼西亚艾尔朗加大学牙科学院微生物研究中心实验室进行。研究在体外进行，样本量使用费德勒公式计算，共四个含有细菌的琼脂平板，每个平板给予五种不同处理：对照组（乙醇）、浓度分别为50%、25%、12.5%和6.25%的蚯蚓提取物（ECT）。获得的以抑制圈直径（以毫米为单位测量）形式的数据使用单因素方差分析进行检验。
治疗组中蚯蚓提取物对牙龈卟啉单胞菌生长的抑制圈平均直径存在显著差异（p<0.05）。对照组与ECT治疗组（ECT 50%、ECT 25%、ECT 12.5%）之间的平均抑制圈在统计学上有差异（p<0.05），相比之下，ECT 6.25%（p>0.05）与对照组无显著差异（p>0.05）。
浓度为50%的蚯蚓提取物对细菌生长的抑制圈直径最大。6.25%的蚯蚓提取物对细菌生长未显示出抗菌活性。
