Konda Prabhakar Yellanur, Dasari Sreenivasulu, Konanki Sreenath, Nagarajan Prabhusaran
Biochemistry Division, National Institute of Nutrition, Hyderabad 500007, India.
Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India.
Heliyon. 2019 Mar 27;5(3):e01373. doi: 10.1016/j.heliyon.2019.e01373. eCollection 2019 Mar.
Abnormal glucose metabolism causes various complications in many metabolic diseases such as obesity, hypertension, cardiovascular diseases and mainly diabetes. But commonly used oral hypoglycemic drugs cause severe side effects. Hence, there is a need to find the medicine without side effects. Compounds of medicinal plants were nontoxic, inexpensive and less or no side effects. Gaertn. (Magenta Cherry) is one of the important medicinal plants in the genus . The fruit of is sour apple-like flavor which tribes using for diabetes without scientific evidence. The purpose of study was to investigate the phytochemical analysis, antihyperglycemic, antihyperlipidemic, antioxidative stress and antioxidant activities of the daily oral administration of the fruit aqueous extract of at a dose of 100 mg/kg bw over a period of 120 days in Streptozotocin-induced diabetic rats. The plant material collected, shade dried and the extracts prepared in increasing order of polarity and screened for different phytoconstituents by Harborne method. The extract with significant antihyperglycemic activity evaluated for antihyperlipidemic, antioxidative stress, antioxidant activity and also for insulin resistance by OGTT. The levels of insulin, HbA1c, lipid profile, glycogen, total proteins, liver and kidney functional markers were measured. The changes in antioxidant defense system were evaluated by TBARS assay. Histopathological examination of pancreas sections carried by hematoxylin and eosin stain. The findings confirm that possesses potential antihyperglycemic, antihyperlipidemic, antioxidative stress and antioxidant activity. The histological changes also coincide with its potential on insulin secretion, glycemic control, lipid metabolisms, oxidative stress and antioxidant enzyme activities. This investigation confirms the traditional use of this plant in the folk medicine.
异常的葡萄糖代谢会在许多代谢性疾病中引发各种并发症，如肥胖症、高血压、心血管疾病，尤其是糖尿病。但常用的口服降糖药会产生严重的副作用。因此，有必要寻找无副作用的药物。药用植物的化合物无毒、价格低廉且副作用少或无副作用。Gaertn.（洋红樱桃）是该属重要的药用植物之一。其果实有类似酸苹果的味道，一些部落将其用于治疗糖尿病，但并无科学依据。本研究的目的是在链脲佐菌素诱导的糖尿病大鼠中，以100mg/kg体重的剂量每日口服该果实水提取物，持续120天，研究其植物化学分析、降血糖、降血脂、抗氧化应激和抗氧化活性。收集植物材料，阴干，按极性递增顺序制备提取物，并采用哈伯恩方法筛选不同的植物成分。对具有显著降血糖活性的提取物进行降血脂、抗氧化应激、抗氧化活性评估，并通过口服葡萄糖耐量试验评估胰岛素抵抗。检测胰岛素、糖化血红蛋白、血脂谱、糖原、总蛋白、肝肾功能指标的水平。通过硫代巴比妥酸反应物（TBARS）测定法评估抗氧化防御系统的变化。用苏木精和伊红染色对胰腺切片进行组织病理学检查。研究结果证实，Gaertn.具有潜在的降血糖、降血脂、抗氧化应激和抗氧化活性。组织学变化也与其在胰岛素分泌、血糖控制、脂质代谢、氧化应激和抗氧化酶活性方面的潜力相符。本研究证实了这种植物在民间医学中的传统用途。
