Motto Aku Enam, Lawson-Evi Povi, Bakoma Batomayena, Eklu-Gadegbeku Kwashie, Aklikokou Kodjo
Laboratory of Physiology/Pharmacology, Faculty of Sciences, University of Lome, BP 1515, Togo.
Department of Pharmacognosy, Faculty of Health Sciences, University of Lome, BP 1515, Togo.
Heliyon. 2021 Apr 2;7(4):e06648. doi: 10.1016/j.heliyon.2021.e06648. eCollection 2021 Apr.
(Combretaceae) is a medicinal plant used in Togo to treat diabetes mellitus and others diseases. The present study was undertaken to evaluate the antihyperlipidemic and antioxidant activities of total extract and fractions of roots of The antihyperlipidemic activity of the total extract and the supernatant was performed by the fructose overload test in ICR mice. Antioxidant potential was determined by methods based on scavenging of DPPH∗, total antioxidant capacity and reducing power. After the screening, phenolic compounds and flavonoids were evaluated by the well-known colorimetric assay using respectively Folin-Ciocalteu reagent and aluminium chloride. The results obtained showed that the total extract and the supernatant significantly reduced the serum and liver levels of triglycerides and hence the level of VLDL-Cholesterol compared to hyperlipidemic mice. the total extract and fractions had the ability to scavenge free radicals, to reduce metal and possessed strong total antioxidant activity. Phytochemical screening revealed the presence of polyphenols, flavonoids, alkaloids, tannins and saponosides in the extract and fractions. And the supernatant fraction contained more polyphenolic compounds than others. From this study, it is concluded that the total extract and fraction of possessed strong antihyperlipidemic, antioxidant properties and were riched in polyphenols, which can be used in the treatment of diabetes mellitus' complications. Hence, the supernatant fraction was the most biologically active.
使君子科植物是多哥用于治疗糖尿病和其他疾病的一种药用植物。本研究旨在评估使君子科植物根的总提取物及其馏分的降血脂和抗氧化活性。通过对ICR小鼠进行果糖过载试验来测定总提取物和上清液的降血脂活性。通过基于清除DPPH∗、总抗氧化能力和还原能力的方法来测定抗氧化潜力。筛选后，分别使用福林-酚试剂和氯化铝通过著名的比色法评估酚类化合物和黄酮类化合物。获得的结果表明，与高脂血症小鼠相比，总提取物和上清液显著降低了甘油三酯的血清和肝脏水平，从而降低了极低密度脂蛋白胆固醇水平。总提取物及其馏分具有清除自由基、还原金属的能力，并具有较强的总抗氧化活性。植物化学筛选显示提取物及其馏分中存在多酚、黄酮、生物碱、单宁和皂苷。并且上清液馏分比其他馏分含有更多的多酚类化合物。从本研究得出结论，使君子科植物的总提取物及其馏分具有较强的降血脂、抗氧化特性，富含多酚，可用于治疗糖尿病并发症。因此，上清液馏分具有最强的生物活性。
