Ishikawa Rie, Fukushima Hotaka, Nakakita Yasukazu, Kado Hisao, Kida Satoshi
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
Frontier Laboratories for Value Creation, Group Research and Development Division, SAPPORO HOLDINGS LTD., Yaizu, Shizuoka, Japan.
Neuropsychopharmacol Rep. 2019 Jun;39(2):140-145. doi: 10.1002/npr2.12054. Epub 2019 Apr 11.
Lactobacillus species are used widely as various food and supplements to improve health. Previous studies have shown that heat-killed Lactobacillus brevis SBC8803 induces serotonin release from intestinal cells and affects sleep rhythm and the autonomic nervous system. However, the effect of SBC8803 on cognitive function remains unknown. Here, we examined the effects of dietary heat-killed SBC8803 on hippocampus-dependent memory and adult hippocampal neurogenesis.
Hippocampus-dependent memory performance was assessed in mice fed heat-killed SBC8803 using social recognition and contextual fear conditioning tasks. Adult hippocampal neurogenesis was evaluated before, during, and after feeding heat-killed SBC8803 by measuring the number of 5-bromo-2-deoxyuridine (BrdU)-positive cells following systemic injections of BrdU using immunohistochemistry.
Mice fed a heat-killed SBC8803 diet showed an improvement of hippocampus-dependent social recognition and contextual fear memories and enhanced adult hippocampal neurogenesis by increasing the survival, but not proliferation, of newborn neurons.
Dietary heat-killed SBC8803 functions as memory and neurogenesis enhancers.
乳酸杆菌属被广泛用作各种食品和补充剂以改善健康。先前的研究表明，热灭活的短乳杆菌SBC8803可诱导肠道细胞释放血清素，并影响睡眠节律和自主神经系统。然而，SBC8803对认知功能的影响尚不清楚。在此，我们研究了饮食中热灭活的SBC8803对海马体依赖性记忆和成年海马体神经发生的影响。
使用社会识别和情境恐惧条件任务，对喂食热灭活SBC8803的小鼠的海马体依赖性记忆表现进行评估。通过免疫组织化学法测量全身注射5-溴-2-脱氧尿苷（BrdU）后BrdU阳性细胞的数量，在喂食热灭活SBC8803之前、期间和之后评估成年海马体神经发生。
喂食热灭活SBC8803饮食的小鼠在海马体依赖性社会识别和情境恐惧记忆方面有所改善，并通过增加新生神经元的存活率而非增殖率，增强了成年海马体神经发生。
饮食中热灭活的SBC8803具有增强记忆和神经发生的作用。
