Department of Chemistry , University of Rome "La Sapienza" , Piazzale Aldo Moro 5 , Rome 00185 , Italy.
J Agric Food Chem. 2019 May 1;67(17):4885-4896. doi: 10.1021/acs.jafc.8b07163. Epub 2019 Apr 19.
Liquid chromatography coupled to low-resolution mass spectrometry (LRMS) has historically been a popular approach for compound quantitation. Recently, high-resolution mass spectrometry (HRMS) technical developments led to the introduction of new approaches for quantitative analysis. Whereas the performances of HRMS have been largely assessed for qualitative purposes, there are still questions about its suitability for quantitative analysis. Several papers on LRMS and HRMS comparison have been published; however, none of them was applied to polyphenol quantitation. In this work, a comparison between HRMS, operated in data-dependent acquisition mode, and LRMS, operated in selected-reaction-monitoring mode, was performed for polyphenol quantitation in wine. The two techniques were evaluated in terms of sensitivity, linearity range, matrix effect, and precision, showing the better performances of HRMS. The suitability of HRMS for quantitation purposes as well as qualitative screening makes HRMS the new technique of choice for both targeted and untargeted analysis.
液相色谱-低分辨质谱（LRMS）在化合物定量方面一直是一种很受欢迎的方法。最近，高分辨质谱（HRMS）技术的发展带来了新的定量分析方法。虽然 HRMS 的性能在很大程度上是为定性目的而评估的，但对于其定量分析的适用性仍存在疑问。已经发表了几篇关于 LRMS 和 HRMS 比较的论文；然而，它们都没有应用于多酚定量。在这项工作中，对葡萄酒中多酚的定量分析进行了 HRMS（数据依赖采集模式）与 LRMS（选择反应监测模式）之间的比较。这两种技术在灵敏度、线性范围、基质效应和精密度方面进行了评估，结果表明 HRMS 的性能更好。HRMS 适合定量目的以及定性筛选，使其成为靶向和非靶向分析的首选新技术。
