Flores Damien, Meudec Emmanuelle, Dias Aécio Luís de Sousa, Sommerer Nicolas
SPO, Université de Montpellier, F-34000 Montpellier, France.
INRAE, Institut Agro, F-34000 Montpellier, France.
Methods Protoc. 2024 Oct 10;7(5):82. doi: 10.3390/mps7050082.
Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.
多酚赋予葡萄酒颜色和涩味，并且作为抗氧化剂，它们还具有有益的健康特性。在这项工作中，我们开发了一种强大的全扫描高分辨率质谱方法，使用超高效液相色谱-轨道阱系统对葡萄酒样品（红葡萄酒、桃红葡萄酒或白葡萄酒）中的90种酚类化合物进行定量分析。通过这种方法，由于亚ppm级的质量精度，我们能够对红葡萄酒、桃红葡萄酒和白葡萄酒中的酚类化合物进行具有高选择性的详细分析。此外，获取全扫描光谱使我们能够监测样品中检测到的所有其他化合物，便于在需要时使该方法适应新的酚类化合物。
