David H L, Rastogi N, Clavel-Sérès S, Clément F
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Sep;262(3):321-34. doi: 10.1016/s0176-6724(86)80005-0.
The antibiotic colistin (polymyxin E) inhibited the lytic cycle of the mycobacteriophage D29 in the tubercle bacilli, but not the D29 adsorption. The protein and nucleic acid synthesis in D29-infected bacteria were not affected significantly. The inhibitory activity was reversed by washing off the antibiotic, and by addition of Ca++, but not in media made iso-osmotic by addition of NaCl or sucrose. Transmission electron microscopy revealed an asymmetric to symmetric transition in the staining profile of the cytoplasmic membrane. Though no mature phage particles were ever observed in colistin-treated, D29-infected tubercle bacilli, loosely arranged aggregates resembling phage proheads were occasionally found. Judging from the above data, it was concluded that colistin inhibited D29 lytic cycle by causing molecular displacements in the inner leaflet of the cytoplasmic membrane, and consequently, the binding sites for D29 structural proteins were not available.
抗生素黏菌素（多黏菌素E）可抑制结核分枝杆菌噬菌体D29在结核杆菌中的裂解周期，但不影响D29的吸附。D29感染细菌中的蛋白质和核酸合成未受到显著影响。通过洗去抗生素以及添加Ca++可逆转抑制活性，但在通过添加NaCl或蔗糖制成等渗的培养基中则不能。透射电子显微镜显示细胞膜染色轮廓从不对称转变为对称。尽管在经黏菌素处理的D29感染结核杆菌中从未观察到成熟的噬菌体颗粒，但偶尔会发现类似噬菌体原头部的松散排列聚集体。根据上述数据得出结论，黏菌素通过引起细胞膜内膜层的分子位移来抑制D29裂解周期，因此，D29结构蛋白的结合位点不可用。
