Bowman B U, Fisher L J, Witiak D T, Newman H A
Am Rev Respir Dis. 1975 Jul;112(1):17-22. doi: 10.1164/arrd.1975.112.1.17.
The adsorption rate constant for mycobacteriophage DS6A on clumped cells of Mycobacterium tuberculosis strain H37Rv was found to be 6.33 x 10(-13) ml per min per colony former. Acetone-extracted cells of H37Rv did not absorb phage DS6A. Acetone extracts of cells of H37Rv and of Mycobacterium bovis strain BCG inhibited plaque formation of mycobacteriophage DS6A and of phage D29. Suspensions of these extracts in heart infusion broth with 0.002 M calcium chloride and 1 per cent dioxane required 20 hours of incubation (activation) at 37 degrees C with stirring before they were capable of inactivating phage DS6A, but did not require activation for inhibition of phage D29. Sonication of water mixtures of these extracts for 45 sec yielded suspensions that were highly active against phage DS6A and independent of the 20-hour lag period. Such preparations were also active against phage D29. The extract prepared from cells of BCG was more active than the extract from cells of H37Rv. Water washing of chloroform solutions of each extract did not remove significant amounts of solid material or reduce the phage-inactivating activites of either extract.
发现分枝杆菌噬菌体DS6A在结核分枝杆菌H37Rv聚集细胞上的吸附速率常数为每分钟每集落形成单位6.33×10⁻¹³毫升。H37Rv的丙酮提取细胞不吸附噬菌体DS6A。H37Rv细胞和牛分枝杆菌卡介苗菌株的丙酮提取物抑制分枝杆菌噬菌体DS6A和噬菌体D29的噬菌斑形成。这些提取物在含有0.002M氯化钙和1%二氧六环的心脏浸液肉汤中的悬浮液，在37℃搅拌下孵育（激活）20小时后才能使噬菌体DS6A失活，但抑制噬菌体D29则不需要激活。将这些提取物的水混合物超声处理45秒，得到的悬浮液对噬菌体DS6A具有高活性，且不受20小时延迟期的影响。这样的制剂对噬菌体D29也有活性。卡介苗细胞制备的提取物比H37Rv细胞制备的提取物活性更高。每种提取物的氯仿溶液水洗后，不会去除大量固体物质，也不会降低任何一种提取物的噬菌体失活活性。
