Lönnerholm G
J Histochem Cytochem. 1980 May;28(5):427-33. doi: 10.1177/28.5.6769996.
Rat liver and rabbit skeletal muscle were studied by Hansson's method for histochemical demonstration of carbonic anhydrase activity. In histochemical model experiments purified male rat liver carbonic anhydrase was much more resistant to acetazolamide than rat erythrocyte carbonic anhydrase. Male rat liver slices showed cytoplasmic staining, which was about 1000 times more resistant to acetazolamide and ethoxzolamide than that of female rat liver or erthyrocytes of either sex. Rabbit skeletal muscle slices showed staining at the sarcolemma of all fibers, whereas the staining of the sarcoplasm varied. The walls of capillaries situated within the muscle bundles were intensely stained. The sarcoplasmic staining of a certain number of fibers was at least 1000 times less sensitive to acetazolamide than the other staining. These findings, which are in good agreement with biochemical data, show that the sulfonamides inhibit histochemical staining in a specific way. This is strong evidence for the specificity of the method.
采用汉森法对大鼠肝脏和兔骨骼肌进行碳酸酐酶活性的组织化学检测。在组织化学模型实验中，纯化的雄性大鼠肝脏碳酸酐酶比大鼠红细胞碳酸酐酶对乙酰唑胺的耐受性更强。雄性大鼠肝脏切片显示胞质染色，其对乙酰唑胺和乙氧唑胺的耐受性比雌性大鼠肝脏或任何性别的红细胞约强1000倍。兔骨骼肌切片显示所有纤维的肌膜均有染色，而肌浆的染色情况各不相同。位于肌束内的毛细血管壁染色强烈。一定数量纤维的肌浆染色对乙酰唑胺的敏感性至少比其他染色低1000倍。这些发现与生化数据高度一致，表明磺胺类药物以特定方式抑制组织化学染色。这是该方法特异性的有力证据。
