Sutera A M, Riggio V, Mastrangelo S, Di Gerlando R, Sardina M T, Pong-Wong R, Tolone M, Portolano B
Dipartimento Scienze Agrarie, Alimentari e Forestali, University of Palermo, 90128, Palermo, Italy.
The Roslin Institute and R(D)SVS, Easter Bush, Roslin, EH25 9RG, UK.
Anim Genet. 2019 Jun;50(3):311-314. doi: 10.1111/age.12789. Epub 2019 Apr 14.
Genome-wide association studies (GWASes) have become a powerful tool for identifying genomic regions associated with important traits in livestock. Milk production traits in dairy sheep are measured at different time points during their life span. Using phenotypic data generated from longitudinal traits could improve the power of association studies but until now have received less attention in GWASes as a methodology and has not been implemented. The aim of this study was to carry out a GWAS for milk production traits in Valle del Belice sheep using repeated measures. After quality control, 469 ewes and 37 228 SNPs were retained for the analysis, and phenotypic data included 5586 test-day records for five milk production traits (milk yield, MY; fat yield and percentage, FY and F%; protein yield and percentage, PY and P%). Nine SNPs located within or close to known genes were found to be associated with milk production traits. In particular, rs398340969, associated with both milk yield and protein yield, is located within the DCPS gene. In addition, rs425417915 and rs417079368, both associated with both fat percentage and protein percentage, are located within the TTC7B gene and at 0.37 Mb within the SUCNR1 gene respectively. In summary, the use of repeated records was beneficial for mapping genomic regions affecting milk production traits in the Valle del Belice sheep.
全基因组关联研究（GWAS）已成为识别与家畜重要性状相关基因组区域的有力工具。奶用绵羊的产奶性状在其生命周期的不同时间点进行测量。利用纵向性状产生的表型数据可以提高关联研究的效力，但迄今为止，作为一种方法，其在GWAS中受到的关注较少，也尚未得到应用。本研究的目的是使用重复测量数据对贝利切山谷绵羊的产奶性状进行全基因组关联研究。经过质量控制后，保留了469只母羊和37228个单核苷酸多态性（SNP）用于分析，表型数据包括五个产奶性状（产奶量，MY；脂肪产量和百分比，FY和F%；蛋白质产量和百分比，PY和P%）的5586条测定日记录。发现9个位于已知基因内部或附近的SNP与产奶性状相关。特别是，与产奶量和蛋白质产量均相关的rs398340969位于DCPS基因内。此外，与脂肪百分比和蛋白质百分比均相关的rs425417915和rs417079368分别位于TTC7B基因内和SUCNR1基因内0.37 Mb处。总之，使用重复记录有助于定位影响贝利切山谷绵羊产奶性状的基因组区域。
