Karigoudar Rashmi M, Karigoudar Mahesh H, Wavare Sanjay M, Mangalgi Smita S
Department of Microbiology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India.
Department of Pathology, BLDE (Deemed to be University) Shri B M Patil Medical College, Vijayapura, Karnataka, India.
J Lab Physicians. 2019 Jan-Mar;11(1):17-22. doi: 10.4103/JLP.JLP_98_18.
accounts for 70%-95% of urinary tract infections (UTIs). UTI is a serious health problem with respect to antibiotic resistance and biofilms formation being the prime cause for the antibiotic resistance. Biofilm can restrict the diffusion of substances and binding of antimicrobials. In this context, the present study is aimed to perform detection of biofilm formation among strains isolated from urine and to correlate their susceptibility pattern with biofilm formation.
A total of 100 strains isolated from patients suffering from UTI were included in the study. The identification of was performed by colony morphology, Gram staining, and standard biochemical tests. The detection of biofilm was carried out by Congo Red Agar (CRA) method, tube method (TM), and tissue culture plate (TCP) method. Antimicrobial sensitivity testing was performed by Kirby-Bauer disc diffusion method on Muller-Hinton agar plate.
Of the 100 strains, 49 (49%) and 51 (51%) were from catheterized and noncatheterized patients, respectively. Biofilm production was positive by CRA, TM, and TCP method were 49 (49%), 55 (55%), and 69 (69%), respectively. Biofilm producers showed maximum resistance to co-trimoxazole (73.9%), gentamicin (94.2%), and imipenem (11.6%) when compared to nonbiofilm producers. Significant association was seen between resistance to antibiotic and biofilm formation with a = 0.01 (<0.05).
A greater understanding of biofilm detection in will help in the development of newer and more effective treatment. The detection of biofilm formation and antibiotic susceptibility pattern helps in choosing the correct antibiotic therapy.
占尿路感染（UTIs）的70%-95%。就抗生素耐药性而言，UTI是一个严重的健康问题，生物膜形成是抗生素耐药性的主要原因。生物膜会限制物质的扩散和抗菌药物的结合。在此背景下，本研究旨在检测从尿液中分离出的菌株中的生物膜形成情况，并将其药敏模式与生物膜形成相关联。
本研究共纳入100株从UTI患者中分离出的菌株。通过菌落形态、革兰氏染色和标准生化试验进行菌株鉴定。通过刚果红琼脂（CRA）法、试管法（TM）和组织培养板（TCP）法进行生物膜检测。在Muller-Hinton琼脂平板上通过 Kirby-Bauer纸片扩散法进行抗菌药敏试验。
在这100株菌株中，49株（49%）和51株（51%）分别来自留置导尿管患者和非留置导尿管患者。通过CRA法、TM法和TCP法检测生物膜产生阳性的分别为49株（49%）、55株（55%）和69株（69%）。与非生物膜产生菌相比，生物膜产生菌对复方新诺明（73.9%）、庆大霉素（94.2%）和亚胺培南（11.6%）的耐药性最高。抗生素耐药性与生物膜形成之间存在显著关联，P = 0.01（<0.05）。
对UTI中生物膜检测的更深入了解将有助于开发更新、更有效的治疗方法。生物膜形成检测和抗生素药敏模式有助于选择正确的抗生素治疗方案。
