Poursina Farkhondeh, Sepehrpour Shima, Mobasherizadeh Sina
Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2018 Mar 27;7:40. doi: 10.4103/abr.abr_116_17. eCollection 2018.
is a Gram-negative, opportunistic human pathogen in which increasing antibiotic resistance is a great concern for continued human survival. Although biofilm formation is a mechanism that helps to survive in unfavorable conditions, according to the importance of biofilm formation in developing the antibiotic resistance here, we studied the relation between antibiotic resistance and qualitative rating method biofilm formation in isolated from patients with urinary tract infection (UTI).
The clinical isolates of ( = 100) were collected from urine of patients with UTI attending Isfahan Alzahra hospital. The strains were confirmed as using biochemical tests and molecular method. The Kirby-Bauer disk diffusion tests were done according to the Clinical and Laboratory Standards Institute protocol, and the biofilm synthesis was performed by microplate method. The binary logistic test was applied and < 0.05 was considered statistically significant.
Our results showed a high outbreak of multidrug-resistant (MDR) strains (73%) and the highest resistance was observed toward ampicillin. The prevalence of biofilm producer isolates was 80% that 29% produced strong biofilm. The distribution of non-MDR isolates was high among strong biofilm producers, which shows a significant negative correlation between biofilm production and MDR pattern ( < 0.001).
We found a negative correlation between MDR phenotype and biofilm formation capacity. This transmits the concept that more antibiotic susceptibility of strong biofilm producers may be due to the reduced exposure to multiple antibiotics.
[病原体名称]是一种革兰氏阴性的机会性人类病原体，其日益增加的抗生素耐药性是人类持续生存的重大担忧。尽管生物膜形成是一种有助于在不利条件下生存的机制，但鉴于生物膜形成在产生抗生素耐药性方面的重要性，我们研究了从尿路感染（UTI）患者分离出的[病原体名称]的抗生素耐药性与生物膜形成定性评级方法之间的关系。
从伊斯法罕阿尔扎赫拉医院就诊的UTI患者尿液中收集了100株[病原体名称]的临床分离株。通过生化试验和分子方法确认这些菌株为[病原体名称]。根据临床和实验室标准协会的方案进行 Kirby-Bauer 纸片扩散试验，并通过微孔板法进行生物膜合成。应用二元逻辑检验，P<0.05被认为具有统计学意义。
我们的结果显示多药耐药（MDR）[病原体名称]菌株的高爆发率（73%），并且观察到对氨苄西林的耐药性最高。生物膜产生菌分离株的患病率为80%，其中29%产生强生物膜。非MDR分离株在强生物膜产生菌中的分布较高，这表明生物膜产生与MDR模式之间存在显著负相关（P<0.001）。
我们发现MDR表型与生物膜形成能力之间存在负相关。这传递了一个概念，即强生物膜产生菌对更多抗生素的敏感性可能是由于较少接触多种抗生素所致。
