Abo-Alella Doaa, Abdelmoniem Wessam, Tantawy Enas, Asaad Ahmed
Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Int Microbiol. 2024 Dec;27(6):1633-1640. doi: 10.1007/s10123-024-00495-w. Epub 2024 Mar 15.
This cross-sectional study aims to determine the incidence and potential risk factors associated with biofilm-producing uropathogenic Escherichia coli (UPEC) nosocomial strains from a tertiary care hospital and to examine the prospective correlation between biofilm generation and antibiotic resistance phenotypes and genotypes.
A total of 130 UPEC nosocomial isolates were identified, their biofilm formation was quantified using a modified microtiter plate assay, and their antibiotic susceptibilities were assessed utilizing the disc diffusion method. Isolates were then subjected to PCR assays targeting bla, bla, bla, and blaOXA48 genes.
Over half of the isolates (n = 76, 58.5%) were biofilm producers. Among 17 carbapenem-resistant isolates, 6 (42.9%) isolates harbored the bla gene, and only 1 (9.1%) isolate was positive for the bla gene. Prior antibiotic therapy (aOR 15.782, p 0.000) and diabetes mellitus DM (aOR 11.222, p 0.016) were the significant risk factors associated with biofilm production, as determined by logistic regression analysis of the data. In addition, gentamicin resistance was the only statistically significant antibiotic resistance pattern associated with biofilm production (aOR 9.113, p 0.02).
The findings of this study emphasize the significance of implementing proper infection control measures to avoid the horizontal spread of biofilm formation and associated antimicrobial resistance patterns among UPEC nosocomial strains.
本横断面研究旨在确定来自一家三级护理医院的产生物膜的医院内致病性大肠埃希菌（UPEC）菌株的发生率及潜在风险因素，并研究生物膜形成与抗生素耐药表型和基因型之间的前瞻性相关性。
共鉴定出130株医院内UPEC分离株，采用改良的微量滴定板法对其生物膜形成进行定量，并利用纸片扩散法评估其抗生素敏感性。然后对分离株进行针对bla、bla、bla和blaOXA48基因的PCR检测。
超过一半的分离株（n = 76，58.5%）产生物膜。在17株耐碳青霉烯类分离株中，6株（42.9%）携带bla基因，仅1株（9.1%）bla基因检测呈阳性。通过对数据进行逻辑回归分析确定，既往抗生素治疗（调整后比值比[aOR] 15.782，p < 0.000）和糖尿病（aOR 11.222，p = 0.016）是与生物膜产生相关的显著风险因素。此外，庆大霉素耐药是与生物膜产生相关的唯一具有统计学意义的抗生素耐药模式（aOR 9.113，p = 0.02）。
本研究结果强调了实施适当感染控制措施以避免UPEC医院内菌株中生物膜形成及相关抗菌药物耐药模式水平传播的重要性。
