Centre d'Excellence en Recherche Nutritionelle, Adisseo SAS, Malicorne, France.
Aix Marseille Univ., CNRS, Centrale Marseille, iSm2, Marseille, France.
Front Immunol. 2019 Mar 29;10:564. doi: 10.3389/fimmu.2019.00564. eCollection 2019.
Strong tight junctions and curtailed inflammatory responses under stressful conditions are key for optimal digestive health. -based probiotics are increasingly being used to maintain broilers' health, but their mode of action is often not well-defined. In the present study we used Caco-2 cells as a model for intestinal epithelia and assessed the effect of three -based probiotics on intestinal barrier function and intestinal inflammation. Experimental results showed that one of the three tested strains, Bs 29784, significantly reinforced intestinal barrier integrity under basal conditions through an up-regulation of the expression of tight junction's proteins, whereas the others had no or detrimental effects. When Caco-2 cells were pre-treated with strains, the subsequent IL-8 release to various pro-inflammatory signals (IL-1β, deoxynivalenol, or flagellin) was blunted compared to cells that had not been pretreated, but to a different extent depending on the strain of used. Bs 29784, was able to significantly decrease IL-8 production in all stressed conditions tested. Mechanistically, Bs 29784 appeared to limit nuclear translocation of NF-κB during IL-1β exposure by preventing IκB degradation. The effects of Bs 29784 were observed independently with supernatant and cells but in a lesser extent than with the combination, indicating that they can thus likely be attributed to both secreted metabolites and cell-associated compounds. Moreover, under inflammatory conditions, Bs 29784 significantly reduced the upregulation of iNOS protein levels further underlining its intestinal anti-inflammatory potential. Our data show that -based probiotics may indeed improve digestive health by strengthening intestinal barrier and limiting inflammatory responses and that these properties are strain-dependent.
在应激条件下，紧密的紧密连接和受限的炎症反应是最佳消化健康的关键。基于益生菌的益生菌越来越多地被用于维持肉鸡的健康，但它们的作用方式通常定义不明确。在本研究中，我们使用 Caco-2 细胞作为肠上皮的模型，评估了三种基于益生菌对肠屏障功能和肠炎症的影响。实验结果表明，在基础条件下，三种测试菌株中的一种，Bs 29784，通过上调紧密连接蛋白的表达，显著增强了肠屏障的完整性，而其他两种则没有或有不利影响。当 Caco-2 细胞用菌株预处理时，与未预处理的细胞相比，随后对各种促炎信号（IL-1β、脱氧雪腐镰刀菌烯醇或鞭毛蛋白）的 IL-8 释放受到抑制，但抑制程度因使用的菌株而异。Bs 29784 能够显著降低所有受应激条件测试的 IL-8 产生。从机制上讲，Bs 29784 通过防止 IκB 降解，似乎能够限制 NF-κB 在 IL-1β 暴露期间的核易位。Bs 29784 的作用是在用上清液和细胞处理时独立观察到的，但程度低于组合处理，这表明它们可能归因于分泌代谢物和细胞相关化合物。此外，在炎症条件下，Bs 29784 显著降低了 iNOS 蛋白水平的上调，进一步强调了其肠道抗炎潜力。我们的数据表明，基于益生菌的益生菌确实可以通过增强肠屏障和限制炎症反应来改善消化健康，并且这些特性是菌株依赖性的。
