Ferguson M, Seagroatt V, Schild G C
J Biol Stand. 1984 Jul;12(3):283-94. doi: 10.1016/s0092-1157(84)80008-6.
The single radial immunodiffusion (SRD) technique has been applied to the assay of the glycoprotein content of rabies vaccines produced in cell cultures. Fourteen laboratories in seven countries participated in a collaborative study to evaluate the reproducibility of the SRD technique; some laboratories also examined vaccines in the mouse protection (NIH) test and by enzyme immunoassay. Good agreement was found between potency estimates using the SRD technique: the geometric coefficients of variation for combined potency estimates of all laboratories were about 10%. SRD assays appear to have a role for the in vitro assay of antigen content of vaccine and could complement results obtained in in vivo assays which are subject to wide variability.
单向辐射免疫扩散（SRD）技术已应用于细胞培养生产的狂犬病疫苗糖蛋白含量的测定。七个国家的14个实验室参与了一项合作研究，以评估SRD技术的重现性；一些实验室还通过小鼠保护（NIH）试验和酶免疫测定法检测了疫苗。使用SRD技术进行的效价估计之间发现了良好的一致性：所有实验室合并效价估计的几何变异系数约为10%。SRD测定似乎可用于疫苗抗原含量的体外测定，并可补充在体内测定中获得的结果，体内测定的结果存在很大差异。
