Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria, Unitat d'Infeccions, Servei de Pediatria, Institut de Recerca Pediàtrica Hospital Sant Joan de Déu, Barcelona, Spain; Departament de Pediatria, Universitat de Barcelona, Barcelona, Spain; CIBER de Epidemiología y Salud Pública, CIBERESP, Madrid, Spain; Red de Investigación Translacional en Infectología Pediátrica, RITIP, Madrid, Spain.
Servei de Microbiologia, Hospital Sant Joan de Déu, Barcelona, Spain.
J Microbiol Methods. 2019 Jun;161:8-11. doi: 10.1016/j.mimet.2019.04.009. Epub 2019 Apr 12.
We assessed the capacity of Kingella kingae to grow in blood culture bottles (BCB), taking into account the concentrations of the microorganism and blood in the culture medium. An initial suspension (McFarland 0.5) of 32 strains of K. kingae was serially diluted. One mL of the initial suspension and 1 mL of the subsequent dilutions were inoculated in two BCB, together with 1 mL of human blood in the 2nd BCB. Also, 1mL serial dilutions of human blood were added to BCBs previously inoculated with 1 mL of K. kingae dilution 1/10. In non-blood-supplemented BCB, 23 strains grew with the initial suspension and only one with the first processed dilution, as compared to all strains with the initial suspension and the 3 first dilutions, 22 with the 4th dilution, and one with the 5th dilution in blood-supplemented BCB. In BCB inoculated with K. kingae dilution 1/10 and decreasing concentrations of human blood, all strains grew with blood dilutions 1/2 and 1/4, 26 with dilution 1/8, 19 with dilution 1/16, 10 with dilution 1/32, and none with dilution 1/64. Increasing time to positivity was observed with both decreasing bacterial (p = .001) and blood concentrations (r = -0.632, p < .0001). The addition of human blood was essential to boost the growth of K. kingae in BCB. If replicated in vivo, these findings would increase the isolation of fastidious K. kingae organisms from pediatric osteoarticular exudates.
我们评估了金氏金氏菌在血培养瓶（BCB）中生长的能力，同时考虑了微生物和培养基中血液的浓度。将 32 株金氏金氏菌的初始悬液（麦氏 0.5）连续稀释。将初始悬液的 1 mL 和后续稀释液的 1 mL 接种到两个 BCB 中，第二个 BCB 中加入 1 mL 人血。此外，将人血的 1 mL 系列稀释液加入先前接种 1 mL 金氏金氏菌稀释度 1/10 的 BCB 中。在未添加血液的 BCB 中，与初始悬液相比，仅 23 株在初始悬液和第一处理稀释液中生长，而所有菌株在初始悬液和前 3 个稀释液中、22 株在第 4 个稀释液中、1 株在第 5 个稀释液中生长，添加血液的 BCB 中。在接种金氏金氏菌稀释度 1/10 和人血浓度逐渐降低的 BCB 中，所有菌株均与人血稀释度 1/2 和 1/4 一起生长，26 株与人血稀释度 1/8 一起生长，19 株与人血稀释度 1/16 一起生长，10 株与人血稀释度 1/32 一起生长，没有人血稀释度 1/64 生长。细菌（p=0.001）和血液浓度（r=-0.632，p<0.0001）的降低均观察到阳性时间延长。如果在体内复制，这些发现将增加从儿科关节渗出物中分离出难养的金氏金氏菌的机会。
