评估五种市售免疫扩散试剂盒用于检测粗球孢子菌和荚膜组织胞浆菌抗体的性能。
Evaluation of five commercially available immunodiffusion kits for detection of Coccidioides immitis and Histoplasma capsulatum antibodies.
作者信息
Johnson J E, Jeffery B, Huppert M
出版信息
J Clin Microbiol. 1984 Sep;20(3):530-2. doi: 10.1128/jcm.20.3.530-532.1984.
Abstract
Five commercial test kits for the serodiagnosis of coccidioidomycosis and histoplasmosis based upon immunodiffusion were evaluated. The correlation of results with the test kits in the Clinical Laboratory varied from 71 to 100% for coccidioidomycosis. The correlation for coccidioidomycosis immunodiffusion testing varied from 57 to 83% when results from the test kits and the Mycology Research Laboratory were compared. Only 81% correlation was noted between the two laboratories when the same reference system was used. Results with the test kits for Histoplasma serodiagnosis and results from the Mycology Research Laboratory showed a correlation of 52 to 75%. There were no false-positive results with any system. All of the commercial kits were 100% specific for the diagnosis of both coccidioidomycosis and histoplasmosis, but the sensitivity of the immunodiffusion tests varied with the system used.
摘要
对五种基于免疫扩散法用于球孢子菌病和组织胞浆菌病血清学诊断的商用检测试剂盒进行了评估。在临床实验室中，这些检测试剂盒对球孢子菌病检测结果的相关性为71%至100%。当将检测试剂盒的结果与真菌学研究实验室的结果进行比较时，球孢子菌病免疫扩散检测的相关性为57%至83%。当使用相同的参考系统时，两个实验室之间的相关性仅为81%。组织胞浆菌病血清学诊断检测试剂盒的结果与真菌学研究实验室的结果显示相关性为52%至75%。任何系统均未出现假阳性结果。所有商用试剂盒对球孢子菌病和组织胞浆菌病的诊断均具有100%的特异性，但免疫扩散试验的敏感性因所使用的系统而异。
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