Wood J C, Friedly G, Zartarian M, Aarnaes S, de la Maza L M
J Clin Microbiol. 1982 Dec;16(6):1030-3. doi: 10.1128/jcm.16.6.1030-1033.1982.
Antibody titers to Coccidioides immitis, using coccidioidin antigen, were determined by three methods: the standardized Laboratory Branch complement fixation method (LBCF), a modified version of the Viral and Rickettsial Disease Laboratory complement fixation test (VRDL-CF), and a quantitative immunodiffusion test (QID). Of the 133 samples evaluated, 72 were negative by each method and 57 (42 serum samples, 15 cerebrospinal fluid samples) were positive by all three methods. Four additional specimens (1 serum sample, 3 cerebrospinal fluid samples) were positive by QID alone. All positive patients were diagnosed clinically as having pulmonary or extrapulmonary coccidioidomycosis or both. When titers from two methods were compared, the agreement within +/- 1 dilution was VRDL-CF/QID, 88.5%; VRDL-CF/LBCF, 85.2%; and LBCF/QID, 82.0%. The agreement of these methods within +/- 2 dilutions was VRDL-CF/QID, 98.4%; VRDL-CF/LBCF, 96.7%; and LBCF/QID, 93.4%. The VRDL-CF and QID methods are simpler to perform; however, they are yet unrecognized as suitable alternatives to the more cumbersome LBCF. Our data show that they should be considered as options for C. immitis serology.
采用球孢子菌素抗原，通过三种方法测定了针对粗球孢子菌的抗体滴度：标准化的实验室分部补体结合试验方法（LBCF）、病毒与立克次体病实验室补体结合试验的改良版本（VRDL - CF）以及定量免疫扩散试验（QID）。在评估的133份样本中，72份样本三种方法检测均为阴性，57份样本（42份血清样本，15份脑脊液样本）三种方法检测均为阳性。另外4份样本（1份血清样本，3份脑脊液样本）仅QID检测呈阳性。所有阳性患者临床诊断均为患有肺球孢子菌病或肺外球孢子菌病或两者皆有。比较两种方法的滴度时，在±1个稀释度内的一致性为：VRDL - CF/QID为88.5%；VRDL - CF/LBCF为85.2%；LBCF/QID为82.0%。这些方法在±2个稀释度内的一致性为：VRDL - CF/QID为98.4%；VRDL - CF/LBCF为96.7%；LBCF/QID为93.4%。VRDL - CF和QID方法操作更简便；然而，它们尚未被视为比更繁琐的LBCF更合适的替代方法。我们的数据表明，它们应被视为粗球孢子菌血清学检测的选择。
