The Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA.
Department of Cellular Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX 78229, USA.
Genes (Basel). 2019 Apr 3;10(4):271. doi: 10.3390/genes10040271.
Fluorescent light (FL) has been utilized for ≈60 years and has become a common artificial light source under which animals, including humans, spend increasing amounts of time. Although the solar spectrum is quite dissimilar in both wavelengths and intensities, the genetic consequences of FL exposure have not been investigated. Herein, we present comparative RNA-Seq results that establish expression patterns within skin, brain, and liver for , , and the hairless mouse () after exposure to FL. These animals represent diurnal and nocturnal lifestyles, and ≈450 million years of evolutionary divergence. In all three organisms, FL induced transcriptional changes of the acute phase response signaling pathway and modulated inflammation and innate immune responses. Our pathway and gene clustering analyses suggest cellular perception of oxidative stress is promoting induction of primary up-stream regulators and . The skin and brain of the three animals as well as the liver of both fish models all exhibit increased inflammation and immune responses; however, the mouse liver suppressed the same pathways. Overall, the conserved nature of the genetic responses observed after FL exposure, among fishes and a mammal, suggest the presence of light responsive genetic circuitry deeply embedded in the vertebrate genome.
荧光灯 (FL) 已使用了约 60 年，并且已经成为动物（包括人类）在其中花费越来越多时间的常见人工光源。尽管太阳光谱在波长和强度上非常不同，但 FL 暴露的遗传后果尚未得到研究。在此，我们提出了比较 RNA-Seq 的结果，这些结果确定了暴露于 FL 后皮肤、大脑和肝脏中的 、 和无毛小鼠 ( ) 的表达模式。这些动物代表了昼夜生活方式和约 4.5 亿年的进化分歧。在所有三种生物中，FL 诱导了急性期反应信号通路的转录变化，并调节了炎症和先天免疫反应。我们的途径和基因聚类分析表明，细胞对氧化应激的感知正在促进主要上游调节剂 和 的诱导。三种动物的皮肤和大脑以及两种鱼类模型的肝脏都表现出炎症和免疫反应增加；然而，小鼠肝脏抑制了相同的途径。总的来说，在鱼类和哺乳动物中观察到的 FL 暴露后的遗传反应的保守性质表明，存在深深嵌入脊椎动物基因组中的光反应性遗传电路。
