School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
School of Pharmacy, Shihezi University (Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education), Shihezi 832001, China.
Int J Mol Sci. 2018 Jun 21;19(7):1822. doi: 10.3390/ijms19071822.
Bai Xuan Xia Ta Re Pian (BXXTR) is a traditional Uighur medicine ancient prescription in China widely used in the treatment of psoriasis, presenting a high curative rate and few side effects. Given that the active constituents and action mechanism still remain unclear, the aim of this study is to explore the potential active constituents and mechanism of antipsoriasis of BXXTR. Psoriasis-like lesions model in BALB/c mice was induced by Imiquimod (IMQ), including five treatment groups: control group, IMQ-treated group, IMQ-ACITRETIN group (Positive control group), IMQ-BXXTR low dose group, IMQ-BXXTR medium dose group and IMQ-BXXTR high dose group. The Psoriasis Area and Severity Index (PASI) score, skin and ear thickness, and histologic section were collected. The differentially expressed genes were determined by using RNAseq technology and the relevant pathways were analyzed by KEGG database. The ELISA kit and western blot assays were used to detect the related protein expression levels. In addition, the chemical constituents of BXXTR were determined by UPLC-TOF-MS analysis and the potential active constituents were predicted by SEA DOCK and Gene Ontology (GO). The data demonstrated that BXXTR significantly alleviated IMQ-induced psoriasis. RNA-seq analysis showed that BXXTR induced the expression levels of 31 genes; the KEGG analysis suggested that BXXTR could significantly change IL-17-related inflammatory pathways. The ELISA kit confirmed that the expression level of IL-17A protein was significantly reduced. 75 compounds of BXXTR were determined by UPLC-TOF-MS analysis, 11 of 75 compounds were identified as potential active compounds by similarity ensemble approach docking (SEA DOCK) and Gene Ontology (GO). BXXTR reduced the severity of skin lesions by inhibiting IL-17-related inflammatory pathways. The results indicated that BXXTR could suppress psoriasis inflammation by multiple-constituents-regulated multiple targets synergistically. Collectively, this study could provide important guidance for the elucidation of the active constituents and action mechanism of BXXTR for the treatment of psoriasis.
Bai Xuan Xia Ta Re Pian（BXXTR）是中国一种传统的维吾尔医药古方，广泛用于治疗银屑病，具有很高的治愈率和很少的副作用。由于其活性成分和作用机制仍不清楚，本研究旨在探索 BXXTR 抗银屑病的潜在活性成分和机制。采用咪喹莫特（IMQ）诱导 BALB/c 小鼠银屑病样病变模型，包括 5 个治疗组：对照组、IMQ 处理组、IMQ-阿维 A 组（阳性对照组）、IMQ-BXXTR 低剂量组、IMQ-BXXTR 中剂量组和 IMQ-BXXTR 高剂量组。采集银屑病面积和严重程度指数（PASI）评分、皮肤和耳朵厚度以及组织切片。采用 RNAseq 技术检测差异表达基因，KEGG 数据库分析相关通路。采用 ELISA 试剂盒和 Western blot 检测相关蛋白表达水平。此外，采用 UPLC-TOF-MS 分析 BXXTR 的化学成分，通过 SEA DOCK 和基因本体论（GO）预测潜在的活性成分。结果表明，BXXTR 显著缓解了 IMQ 诱导的银屑病。RNA-seq 分析显示，BXXTR 诱导了 31 个基因的表达水平；KEGG 分析表明，BXXTR 可显著改变 IL-17 相关炎症通路。ELISA 试剂盒证实，IL-17A 蛋白的表达水平显著降低。通过 UPLC-TOF-MS 分析确定了 BXXTR 的 75 种化合物，通过相似性集成方法对接（SEA DOCK）和基因本体论（GO）确定了 75 种化合物中的 11 种为潜在的活性化合物。BXXTR 通过抑制 IL-17 相关炎症通路减轻皮肤病变的严重程度。结果表明，BXXTR 可以通过多成分调节多靶点协同抑制银屑病炎症。综上所述，本研究可为阐明 BXXTR 治疗银屑病的活性成分和作用机制提供重要指导。
