EA4483-IMPECS, Institut Pasteur de Lille, Université de Lille, CHU Lille, 59045 Lille CEDEX, France.
Département Sciences de l'Atmosphère et Génie de l'Environnement (SAGE), IMT Lille Douai, Université de Lille, 59000 Lille, France.
Int J Environ Res Public Health. 2019 Apr 4;16(7):1210. doi: 10.3390/ijerph16071210.
Exposure to particulate matter (PM) is leading to various respiratory health outcomes. Compared to coarse and fine particles, less is known about the effects of chronic exposure to ultrafine particles, despite their higher number and reactivity. In the present study, we performed a time-course experiment in mice to better analyze the lung impact of atmospheric ultrafine particles, with regard to the effects induced by fine particles collected on the same site. Trace element and PAH analysis demonstrated the almost similar chemical composition of both particle fractions. Mice were exposed intranasally to FF or UFP according to acute (10, 50 or 100 µg of PM) and repeated (10 µg of PM 3 times a week during 1 or 3 months) exposure protocols. More particle-laden macrophages and even greater chronic inflammation were observed in the UFP-exposed mice lungs. Histological analyses revealed that about 50% of lung tissues were damaged in mice exposed to UFP for three months versus only 35% in FF-exposed mice. These injuries were characterized by alveolar wall thickening, macrophage infiltrations, and cystic lesions. Taken together, these results strongly motivate the update of current regulations regarding ambient PM concentrations to include UFP and limit their emission.
暴露于颗粒物（PM）会导致各种呼吸道健康问题。与粗颗粒物和细颗粒物相比，人们对慢性暴露于超细颗粒物的影响知之甚少，尽管超细颗粒物的数量更多且反应性更强。在本研究中，我们在小鼠中进行了时间过程实验，以更好地分析大气超细颗粒物对肺的影响，同时考虑了在同一地点收集的细颗粒物所诱导的影响。微量元素和 PAH 分析表明，这两种颗粒的化学组成几乎相似。根据急性（10、50 或 100µg 的 PM）和重复（每周 3 次，每次 10µg 的 PM，持续 1 或 3 个月）暴露方案，通过鼻腔内暴露于 FF 或 UFP。在 UFP 暴露的小鼠肺部中观察到更多载有颗粒的巨噬细胞和更大的慢性炎症。组织学分析显示，暴露于 UFP 三个月的小鼠中约有 50%的肺组织受损，而暴露于 FF 的小鼠中只有 35%。这些损伤的特征是肺泡壁增厚、巨噬细胞浸润和囊性病变。总之，这些结果强烈促使更新当前有关环境 PM 浓度的法规，将 UFP 包括在内并限制其排放。
