枯草芽孢杆菌prtR基因的特性鉴定与定位
Characterization and mapping of the Bacillus subtilis prtR gene.
作者信息
Yang M, Shimotsu H, Ferrari E, Henner D J
出版信息
J Bacteriol. 1987 Jan;169(1):434-7. doi: 10.1128/jb.169.1.434-437.1987.
Abstract
A gene from Bacillus natto encoding a 60-amino-acid peptide has been previously described that, when cloned on a high-copy plasmid in B. subtilis, enhances production of alkaline protease, neutral protease, and levansucrase. An identical gene was isolated from B. subtilis and caused a similar phenotype when placed on a high-copy plasmid. Genetic mapping localized this gene near metB, distant from other pleiotropic genes causing similar effects. Deletion of this gene from the B. subtilis chromosome had no obvious phenotypic effect.
摘要
先前已描述了一种来自纳豆芽孢杆菌的基因，其编码一个60个氨基酸的肽，当该基因克隆到枯草芽孢杆菌的高拷贝质粒上时，可提高碱性蛋白酶、中性蛋白酶和蔗糖转化酶的产量。从枯草芽孢杆菌中分离出一个相同的基因，当置于高拷贝质粒上时会产生相似的表型。遗传图谱将该基因定位在metB附近，与引起类似效应的其他多效性基因距离较远。从枯草芽孢杆菌染色体上缺失该基因没有明显的表型效应。
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