枯草芽孢杆菌的高产蛋白酶突变体。
Hyperprotease-producing mutants of Bacillus subtilis.
作者信息
Higerd T B, Hoch J A, Spizizen J
出版信息
J Bacteriol. 1972 Nov;112(2):1026-8. doi: 10.1128/jb.112.2.1026-1028.1972.
Abstract
A number of mutants of Bacillus subtilis producing high levels of extracellular protease have been isolated. Analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. The elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. All of the mutants genetically analyzed were found linked to the argC4 marker by PBS-1 transduction analysis.
摘要
已分离出许多产生高水平胞外蛋白酶的枯草芽孢杆菌突变体。对这些突变体培养上清液的分析表明，蛋白水解活性总量比野生菌株提高了16至37倍。活性升高是由于中性和碱性蛋白酶同时增加。通过PBS-1转导分析发现，所有经遗传分析的突变体都与argC4标记有关。
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