Midda M, Cooksey M W
J Clin Periodontol. 1986 Nov;13(10):950-6. doi: 10.1111/j.1600-051x.1986.tb01433.x.
Previous studies have shown that the inclusion of certain enzymes in mouthrinses and dentifrices will reduce plaque and gingivitis scores. The enzymes that are most effective clinically have, as their active ingredients, amyloglucosidase and glucose oxidase. These produce hydrogen peroxide from dietary fermentable carbohydrates which in turn converts thiocyanate to hypothiocyanite in the presence of salivary lactoperoxidase. The resultant hypothiocyanite acts as a bacterial inhibitor by interfering with cell metabolism; thus, there is a reduction in plaque accumulation and therefore in gingival inflammation. Pilot studies have compared over a short period the action of the trial dentifrice with enzymes and fluoride at 1100 ppm, using as controls the paste without enzymes but with fluoride and a commercial fluoride paste. There was an expected reduction in all scores with all products due to the mechanical removal of plaque, but a significantly greater reduction in gingivitis was noted in the paste with enzymes. This study is of longer duration with many more subjects. Baseline data include plaque and gingival indices and Periotron readings for crevicular fluid. The trial is of a double-blind non-crossover study design using a split-mouth technique. One side of the mouth is given a prophylaxis and the subject given one of the 3 test pastes to use. Readings were repeated every 2 weeks for 3 months. The results show a significant reduction in gingivitis scores in the enzyme-containing dentifrice group.
先前的研究表明，在漱口水和牙膏中添加某些酶会降低牙菌斑和牙龈炎评分。临床上最有效的酶，其活性成分是淀粉葡萄糖苷酶和葡萄糖氧化酶。这些酶从膳食中可发酵的碳水化合物产生过氧化氢，过氧化氢在唾液乳过氧化物酶存在的情况下又将硫氰酸盐转化为次硫氰酸盐。生成的次硫氰酸盐通过干扰细胞代谢起到细菌抑制剂的作用；因此，牙菌斑的积累减少，进而牙龈炎症减轻。初步研究在短时间内比较了含酶试验牙膏与含1100 ppm氟化物牙膏的作用，使用不含酶但含氟化物的牙膏和市售含氟牙膏作为对照。由于机械去除牙菌斑，所有产品的各项评分都出现了预期的降低，但含酶牙膏中牙龈炎的降低更为显著。本研究持续时间更长，受试者更多。基线数据包括牙菌斑和牙龈指数以及龈沟液的牙周探针读数。该试验采用双盲非交叉研究设计，使用分口技术。口腔一侧进行预防性清洁，受试者使用三种测试牙膏中的一种。在3个月内每2周重复进行读数。结果显示，含酶牙膏组的牙龈炎评分显著降低。
