Department of Cell and Molecular Biology, Microbiology, Uppsala University, P.O. Box 596, 751 24, Uppsala, Sweden.
Department of Ecology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7044, 750 07, Uppsala, Sweden.
Microb Ecol. 2019 Nov;78(4):781-791. doi: 10.1007/s00248-019-01369-9. Epub 2019 Apr 15.
The microbiota in mosquito breeding waters can affect ovipositing mosquitoes, have effects on larval development, and can modify adult mosquito-gut bacterial composition. This, in turn, can affect transmission of human pathogens such as malaria parasites. Here, we explore the microbiota of four breeding sites for Anopheles darlingi, the most important malaria vector in Latin America. The sites are located in Manaus in the Amazon basin in Brazil, an area of active malaria transmission. Using 16S rRNA gene sequencing by MiSeq, we found that all sites were dominated by Proteobacteria and Firmicutes and that 94% of the total number of reads belonged to 36 operational taxonomic units (OTUs) identified in all sites. Of these, the most common OTUs belonged to Escherichia/Shigella, Staphylococcus, and Pseudomonas. Of the remaining 6% of the reads, the OTUs found to differentiate between the four sites belonged to the orders Burkholderiales, Actinomycetales, and Clostridiales. We conclude that An. darlingi can develop in breeding waters with different surface-water bacteria, but that the common microbiota found in all breeding sites might indicate or contribute to a suitable habitat for this important malaria vector.
蚊媒滋生地的微生物群会影响产卵的蚊子，对幼虫发育产生影响，并能改变成年蚊子肠道中的细菌组成。这反过来又会影响疟疾寄生虫等人类病原体的传播。在这里，我们探索了拉丁美洲最重要的疟疾传播媒介按蚊 Darlingi 的四个滋生地的微生物群。这些地点位于巴西亚马逊盆地的马瑙斯，该地区有活跃的疟疾传播。使用 MiSeq 的 16S rRNA 基因测序，我们发现所有地点都以变形菌门和厚壁菌门为主，总读数的 94%属于在所有地点都能识别的 36 个操作分类单元（OTU）。其中，最常见的 OTU 属于大肠杆菌/志贺氏菌、葡萄球菌和假单胞菌。在其余 6%的读取中，区分四个地点的 OTU 属于伯克霍尔德氏菌目、放线菌目和梭菌目。我们得出结论，按蚊 Darlingi 可以在具有不同地表水细菌的滋生地中发育，但在所有滋生地中发现的常见微生物群可能表明或有助于为这种重要的疟疾传播媒介提供合适的栖息地。
