Huang Ting, Xie Wei, Liu Xiao-Dong, Tang Kai-Xun, Yang Rui
College of Environment, Chengdu University of Technology Chengdu 610059, China.
College of Materials, Chemistry & Chemical Engineering, Chengdu University of Technology Chengdu 610059, China.
Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(3):460-464. doi: 10.19540/j.cnki.cjcmm.20181204.007.
In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.
为了寻找新型抗真菌剂来源，采用传统方法从白屈菜的根、茎和叶中分离出11株可培养内生真菌。通过形态学特征和分子生物学技术，其中7株被鉴定为炭疽菌属（L1、L2、L3、S1、S3、S4、S5），3株被鉴定为镰刀菌属（R1、R2、R3）。抗真菌活性测试表明，所有受试真菌对5种常见植物病原菌（胶孢炭疽菌、新月弯孢菌、稻瘟病菌、链格孢菌和芸苔链格孢菌）均表现出一定的抑制活性，部分测试项目的抑制率超过60%。其中，R1、S2、S3和S4的活性比其他菌株更强。本研究丰富了对白屈菜内生菌的认识，为新型微生物杀菌剂研究提供了依据。
