Zhou Chun-Yuan, Piao Xiang-Min, Yan Mei-Xia, Wang Ying-Ping
Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences Changchun 130112,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jan;44(2):274-277. doi: 10.19540/j.cnki.cjcmm.20181106.003.
To obtain biocontrol fungus for Alternaria panax,the antifungal effects of one strain of endophytic fungi isolated from leaves of healthy ginseng were screened and evaluated by using dual-culture method,and the taxonomic assignment of the screened strain was identified based on the morphological characters and ITS sequence analysis. The results of dual-culture showed that one of the endophytes marked as FS-01 had good antifungal effects and the inhibitory rates of FS-01 strain to A. panax was( 60. 21±0. 12) %.The hyphae junction of the both strains,A. panax dissolved,broke and winded,while the hyphae of FS-01 strain remained normal. The inhibitory rates of non-sterilized FS-01 strain fermentation liqud was( 13. 94±0. 21) %. Strain FS-01 identified as Chaetomium globosum.
为获得人参链格孢的生防真菌，采用对峙培养法对从健康人参叶片中分离得到的1株内生真菌的抑菌效果进行筛选和评价，并基于形态特征和ITS序列分析对筛选出的菌株进行分类鉴定。对峙培养结果表明，标记为FS-01的1株内生菌具有良好的抑菌效果，FS-01菌株对人参链格孢的抑制率为(60.21±0.12)%。两菌株（人参链格孢和FS-01）的菌丝交接处，人参链格孢的菌丝溶解、断裂并缠绕，而FS-01菌株的菌丝保持正常。未灭菌的FS-01菌株发酵液的抑制率为(13.94±0.21)%。菌株FS-01鉴定为球毛壳菌。
