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黄连内生细菌的分离与鉴定

Isolation and characterization of bacterial endophytes of Chelidonium majus L.

机构信息

Department of Microbial Biology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Poland.

出版信息

Pol J Microbiol. 2009;58(4):355-61.

Abstract

The aim of this study was to isolate and identify endophytic bacteria from stems of Chelidonium majus L. (greater celandine) and to evaluate their antifungal properties. In total, 34 bacterial endophyte strains were isolated. The fungistatic effects of these bacteria on the growth of five moulds (Alternaria alternata, Chaetonium sp., Paecilomyces variotti, Byssochlamys fulva, Aureobasidium pullulans) and one species of black yeast (Exophiala mesophila) were tested. The majority of the bacterial isolates were found to inhibit the growth of fungi and those with the strongest antifungal properties were further characterized. Of the twelve isolates examined, 11 were species of Bacillus thuringiensis and one was Bacillus amyloliquefaciens.

摘要

本研究旨在从Chelidonium majus L.(蒲公英)的茎部分离和鉴定内生细菌,并评估其抗真菌特性。总共分离出 34 株细菌内生菌株。这些细菌对五种霉菌(Alternaria alternata、Chaetonium sp.、Paecilomyces variotti、Byssochlamys fulva、Aureobasidium pullulans)和一种黑酵母(Exophiala mesophila)的生长具有抑菌作用。发现大多数细菌分离株能抑制真菌的生长,对具有最强抗真菌特性的分离株进行了进一步的特征描述。在所检查的 12 个分离株中,有 11 个是苏云金芽孢杆菌的种,一个是解淀粉芽孢杆菌的种。

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