纳米颗粒支撑脂质体中膜蛋白的单向呈现。
Unidirectional Presentation of Membrane Proteins in Nanoparticle-Supported Liposomes.
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA, 02115, USA.
Division of Molecular Medicine, Boston Children's Hospital, Department of Pediatrics, Harvard Medical School, 3 Blackfan Street, Boston, MA, 02115, USA.
出版信息
Angew Chem Int Ed Engl. 2019 Jul 15;58(29):9866-9870. doi: 10.1002/anie.201903093. Epub 2019 May 7.
Abstract
Presentation of membrane proteins to host immune systems has been a challenging problem owing to complexity arising from the poor in vivo stability of the membrane-mimetic media often used for solubilizing the membrane proteins. The use of functionalized, biocompatible nanoparticles as substrates is shown to guide the formation of proteoliposomes, which can present many copies of membrane proteins in a unidirectional manner. The approach was demonstrated to present the membrane-proximal region of the HIV-1 envelope glycoprotein. These nanoparticle-supported liposomes are broadly applicable as membrane antigen vehicles for inducing host immune responses.
摘要
由于用于溶解膜蛋白的膜模拟介质的体内稳定性差而导致的复杂性，向宿主免疫系统呈现膜蛋白一直是一个具有挑战性的问题。使用功能化的、生物相容性的纳米粒子作为基底被证明可以指导形成脂质体，这些脂质体可以以单向的方式呈现许多膜蛋白的拷贝。该方法被证明可以呈现 HIV-1 包膜糖蛋白的膜近端区域。这些纳米粒子支撑的脂质体作为膜抗原载体广泛适用于诱导宿主免疫反应。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):E8892-E8899. doi: 10.1073/pnas.1807259115. Epub 2018 Sep 5.
2
Potent and broad HIV-neutralizing antibodies in memory B cells and plasma.记忆B细胞和血浆中强效且广谱的HIV中和抗体。
Sci Immunol. 2017 Jan 27;2(7). doi: 10.1126/sciimmunol.aal2200.
3
Structural basis for membrane anchoring of HIV-1 envelope spike.HIV-1包膜刺突膜锚定的结构基础。
Science. 2016 Jul 8;353(6295):172-175. doi: 10.1126/science.aaf7066. Epub 2016 Jun 23.
4
Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs.利用纳米圆盘抗体噬菌体展示技术进行膜蛋白结构研究的构象伴侣蛋白
Structure. 2016 Feb 2;24(2):300-9. doi: 10.1016/j.str.2015.11.014. Epub 2015 Dec 31.
5
Phage display selections for affinity reagents to membrane proteins in nanodiscs.用于筛选与纳米圆盘膜蛋白结合的亲和试剂的噬菌体展示技术。
Methods Enzymol. 2015;557:219-45. doi: 10.1016/bs.mie.2014.12.032. Epub 2015 Mar 24.
6
Design of lipid nanocapsule delivery vehicles for multivalent display of recombinant Env trimers in HIV vaccination.用于HIV疫苗接种中重组Env三聚体多价展示的脂质纳米胶囊递送载体的设计
Bioconjug Chem. 2014 Aug 20;25(8):1470-8. doi: 10.1021/bc5002246. Epub 2014 Jul 14.
7
Engineering multifunctional capsules through the assembly of metal-phenolic networks.通过金属-酚醛网络的组装来构建多功能胶囊。
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9
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J Virol. 2014 Jan;88(2):1249-58. doi: 10.1128/JVI.02664-13. Epub 2013 Nov 13.
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