a Advanced Magnetic Materials Research Center, College of Engineering , University of Tehran , Tehran , Iran.
b Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute , ACECR , Tehran , Iran.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1437-1443. doi: 10.1080/21691401.2019.1596939.
Tumour drug delivery using nanocarriers is attracting ample attentions due to their high drug-loading capacity. Regarding specific tumour microenvironment properties as acidic pH, smart nanocarriers with the ability of responding to the microenvironment, can have a profound effect on the level of drug release and subsequent tumour treatment. In this study, by combining the advantages of multiwall carbon nanotube and pH-sensitive nanogels, multifunctional magneto/pH-responsive nano-hybrid system is developed to deliver the doxorubicin as a general cancer chemotherapeutic drug. The chemical and physical properties of the nanocarrier, as well as drug-loading efficiency and drug releasing characteristics were analysed. It was showed functionalized CNT has low pH-responsiveness in acidic environment, whereas chitosan-coated magnetic nanocomposite can result in greater pH-responsiveness and subsequently higher drug release over a week compared to nanocomposite system without chitosan. This behaviour was proved in Live/Dead assay of the U-87 glioblastoma cell lines exposed to DOX release supernatant at different time intervals so that significant effect of DOX supernatant on cancer cell proliferation suppression was showed.
由于纳米载体具有较高的载药能力，因此利用其进行肿瘤药物输送引起了广泛关注。针对特定的肿瘤微环境特性（如酸性 pH 值），具有响应微环境能力的智能纳米载体可以对药物释放水平和随后的肿瘤治疗产生深远影响。在这项研究中，通过结合多壁碳纳米管和 pH 敏感纳米凝胶的优势，开发了多功能磁/pH 响应纳米杂化系统，以阿霉素作为一般的癌症化疗药物进行输送。分析了纳米载体的化学和物理性质、载药效率和药物释放特性。结果表明，功能化 CNT 在酸性环境中具有较低的 pH 响应性，而壳聚糖包覆的磁性纳米复合材料与没有壳聚糖的纳米复合材料系统相比，在一周内可以产生更大的 pH 响应性，从而实现更高的药物释放。这一行为通过 U-87 神经胶质瘤细胞系在不同时间间隔暴露于 DOX 释放上清液的 Live/Dead 测定得到了证实，结果表明 DOX 上清液对癌细胞增殖抑制有显著作用。
