Amelioration of PM-induced lung toxicity in rats by nutritional supplementation with fish oil and Vitamin E.

BACKGROUND

Exposure to fine particulate matter (PM) has been associated with respiratory morbidity and mortality. Identification of interventional measures that are efficacious against PM-induced toxicity may provide public health benefits. This study examined the inhibitory effects of nutritional supplementation with fish oil as a source of omega-3 fatty acids and vitamin E (Vit E) on PM-induced lung toxicity in rats.

METHOD

Sixty four male Sprague Dawley rats were gavaged with phosphate buffered saline (PBS), corn oil (5 ml/kg), fish oil (150 mg/kg), or Vit E (75 mg/kg), respectively, once a day for 21 consecutive days prior to intratracheal instillation of PM (10 mg/kg) every other day for a total of 3 times. Serum and bronchoalveolar lavage fluids (BALFs) were collected 24 h after the last instillation of PM. Levels of total proteins (TP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), 8-epi-prostaglandin F2α (8-epi-PGF2α), interleukin-1β (IL-1β), C-reactive protein (CRP), IL-6, and tumor necrosis factor-ɑ (TNF-ɑ) were analyzed for markers of cell injury and inflammation. Additionally, histological alterations of lung tissues were examined by hematoxylin-eosin staining.

RESULT

Exposure to PM resulted in lung toxicity, represented as increased levels of total proteins, LDH, 8-epi-PGF2α, IL-1β and TNF-α, and increased infiltration of inflammatory cells, and decreased SOD in the BALFs, and systemic inflammation, as evinced by increased levels of CRP and IL-6 in serum. Strikingly, supplementation with fish oil but not Vit E significantly ameliorated PM-induced lung toxicity and systemic inflammation.

CONCLUSION

PM exposure induces oxidative stress, lung injury and inflammation, which is ameliorated significantly by fish oil and partially by Vit E.